Stable deuterium internal standard for the isotope-dilution LC-MS/MS analysis of elastin degradation

Shuren Ma; Gerard M. Turino; Takahiro Hayashi; Hiroto Yanuma; Toyonobu Usuki; Yong Y. Lin

doi:10.1016/j.ab.2013.05.014

Stable deuterium internal standard for the isotope-dilution LC-MS/MS analysis of elastin degradation

Shuren Ma, Gerard M. Turino, Takahiro Hayashi, Hiroto Yanuma, Toyonobu Usuki, Yong Y. Lin

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Chemical synthesis of the deuterium isotope desmosine-d₄ has been achieved. This isotopic compound possesses all four deuterium atoms at the alkanyl carbons of the alkyl amino acid substitution in the desmosine molecule and is stable toward acid hydrolysis; this is required in the measurement of two crosslinking molecules, desmosine and isodesmosine, as biomarkers of elastic tissue degradation. The degradation of elastin occurs in several widely prevalent diseases. The synthesized desmosine-d4 is used as the internal standard to develop an accurate and sensitive isotope-dilution liquid chromatography- tandem mass spectrometry analysis, which can serve as a generalized method for an accurate analysis of desmosine and isodesmosine as biomarkers in many types of biological tissues involving elastin degradation.

Original language	English
Pages (from-to)	158-165
Number of pages	8
Journal	Analytical Biochemistry
Volume	440
Issue number	2
DOIs	https://doi.org/10.1016/j.ab.2013.05.014
State	Published - 2013
Externally published	Yes

Keywords

Biomarkers of elastin degradation
Desmosine
Isodesmosine
Isotope-dilution LC-MS/MS

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10.1016/j.ab.2013.05.014

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title = "Stable deuterium internal standard for the isotope-dilution LC-MS/MS analysis of elastin degradation",

abstract = "Chemical synthesis of the deuterium isotope desmosine-d4 has been achieved. This isotopic compound possesses all four deuterium atoms at the alkanyl carbons of the alkyl amino acid substitution in the desmosine molecule and is stable toward acid hydrolysis; this is required in the measurement of two crosslinking molecules, desmosine and isodesmosine, as biomarkers of elastic tissue degradation. The degradation of elastin occurs in several widely prevalent diseases. The synthesized desmosine-d4 is used as the internal standard to develop an accurate and sensitive isotope-dilution liquid chromatography- tandem mass spectrometry analysis, which can serve as a generalized method for an accurate analysis of desmosine and isodesmosine as biomarkers in many types of biological tissues involving elastin degradation.",

keywords = "Biomarkers of elastin degradation, Desmosine, Isodesmosine, Isotope-dilution LC-MS/MS",

author = "Shuren Ma and Turino, {Gerard M.} and Takahiro Hayashi and Hiroto Yanuma and Toyonobu Usuki and Lin, {Yong Y.}",

note = "Funding Information: This work was supported by funds from James P. Mara Center for Lung Diseases, the Flight Attendant Medical Research Institute, the Charles A. Mastronardi Foundation, the Ned Doyle Foundation, the Alpha-1 Foundation, and by funds from Ethel Kennedy, John Kennedy, and Judith Sulzberger. This work is also supported by funds (to T.U.) from a Grant-in-Aid for Young Scientists (B) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (KAKENHI Grant 22710224); the Shimadzu Science Foundation; the SEI Group CSR Foundation; and the Naito Science Foundation. ",

year = "2013",

doi = "10.1016/j.ab.2013.05.014",

language = "English",

volume = "440",

pages = "158--165",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "2",

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T1 - Stable deuterium internal standard for the isotope-dilution LC-MS/MS analysis of elastin degradation

AU - Ma, Shuren

AU - Turino, Gerard M.

AU - Hayashi, Takahiro

AU - Yanuma, Hiroto

AU - Usuki, Toyonobu

AU - Lin, Yong Y.

N1 - Funding Information: This work was supported by funds from James P. Mara Center for Lung Diseases, the Flight Attendant Medical Research Institute, the Charles A. Mastronardi Foundation, the Ned Doyle Foundation, the Alpha-1 Foundation, and by funds from Ethel Kennedy, John Kennedy, and Judith Sulzberger. This work is also supported by funds (to T.U.) from a Grant-in-Aid for Young Scientists (B) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (KAKENHI Grant 22710224); the Shimadzu Science Foundation; the SEI Group CSR Foundation; and the Naito Science Foundation.

PY - 2013

Y1 - 2013

N2 - Chemical synthesis of the deuterium isotope desmosine-d4 has been achieved. This isotopic compound possesses all four deuterium atoms at the alkanyl carbons of the alkyl amino acid substitution in the desmosine molecule and is stable toward acid hydrolysis; this is required in the measurement of two crosslinking molecules, desmosine and isodesmosine, as biomarkers of elastic tissue degradation. The degradation of elastin occurs in several widely prevalent diseases. The synthesized desmosine-d4 is used as the internal standard to develop an accurate and sensitive isotope-dilution liquid chromatography- tandem mass spectrometry analysis, which can serve as a generalized method for an accurate analysis of desmosine and isodesmosine as biomarkers in many types of biological tissues involving elastin degradation.

AB - Chemical synthesis of the deuterium isotope desmosine-d4 has been achieved. This isotopic compound possesses all four deuterium atoms at the alkanyl carbons of the alkyl amino acid substitution in the desmosine molecule and is stable toward acid hydrolysis; this is required in the measurement of two crosslinking molecules, desmosine and isodesmosine, as biomarkers of elastic tissue degradation. The degradation of elastin occurs in several widely prevalent diseases. The synthesized desmosine-d4 is used as the internal standard to develop an accurate and sensitive isotope-dilution liquid chromatography- tandem mass spectrometry analysis, which can serve as a generalized method for an accurate analysis of desmosine and isodesmosine as biomarkers in many types of biological tissues involving elastin degradation.

KW - Biomarkers of elastin degradation

KW - Desmosine

KW - Isodesmosine

KW - Isotope-dilution LC-MS/MS

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DO - 10.1016/j.ab.2013.05.014

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AN - SCOPUS:84884913470

SN - 0003-2697

VL - 440

SP - 158

EP - 165

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Stable deuterium internal standard for the isotope-dilution LC-MS/MS analysis of elastin degradation

Abstract

Keywords

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