Spray-dried powders of starch and crosslinked poly(acrylic acid) as carriers for nasal delivery of inactivated influenza vaccine

D. Coucke; M. Schotsaert; C. Libert; E. Pringels; C. Vervaet; P. Foreman; X. Saelens; J. P. Remon

doi:10.1016/j.vaccine.2008.12.013

Spray-dried powders of starch and crosslinked poly(acrylic acid) as carriers for nasal delivery of inactivated influenza vaccine

D. Coucke, M. Schotsaert, C. Libert, E. Pringels, C. Vervaet, P. Foreman, X. Saelens, J. P. Remon

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

Mucosal vaccination has several advantages over parenteral vaccination. In this study, viscosity-enhancing mucosal delivery systems for the induction of an adaptive immune response against viral antigen were investigated. Powder formulations based on spray-dried mixtures of starch (Amioca^®)/poly(acrylic acid) (Carbopol^® 974P) in different ratios were used as carriers of the viral antigen. A comparison of these formulations for intranasal delivery of heat-inactivated influenza virus combined with LTR192G adjuvant was made in vivo in a rabbit model. Individual rabbit sera were tested for seroconversion against hemagglutinin (HA), the major surface antigen of influenza. The powder vaccine formulations were able to induce systemic anti-HA IgG responses. The presence of Carbopol^® 974P improved the kinetics of the immune responses and the level of IgG titers in a dose-dependent way which was correlated with moderately irritating capacities of the formulation. In contrast, mucosal IgA responses were not detected. In conclusion, it was demonstrated that the use of bioadhesive carriers based on Amioca^® starch and poly(acrylic acid) facilitates the induction of a systemic anti-HA antibody response after intranasal vaccination with a whole virus influenza vaccine.

Original language	English
Pages (from-to)	1279-1286
Number of pages	8
Journal	Vaccine
Volume	27
Issue number	8
DOIs	https://doi.org/10.1016/j.vaccine.2008.12.013
State	Published - 18 Feb 2009
Externally published	Yes

Keywords

Muco-adhesion
Nasal delivery
Nasal vaccination
Powder formulation

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10.1016/j.vaccine.2008.12.013

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@article{0f6a7fa861df413680eb928f48b1f476,

title = "Spray-dried powders of starch and crosslinked poly(acrylic acid) as carriers for nasal delivery of inactivated influenza vaccine",

abstract = "Mucosal vaccination has several advantages over parenteral vaccination. In this study, viscosity-enhancing mucosal delivery systems for the induction of an adaptive immune response against viral antigen were investigated. Powder formulations based on spray-dried mixtures of starch (Amioca{\textregistered})/poly(acrylic acid) (Carbopol{\textregistered} 974P) in different ratios were used as carriers of the viral antigen. A comparison of these formulations for intranasal delivery of heat-inactivated influenza virus combined with LTR192G adjuvant was made in vivo in a rabbit model. Individual rabbit sera were tested for seroconversion against hemagglutinin (HA), the major surface antigen of influenza. The powder vaccine formulations were able to induce systemic anti-HA IgG responses. The presence of Carbopol{\textregistered} 974P improved the kinetics of the immune responses and the level of IgG titers in a dose-dependent way which was correlated with moderately irritating capacities of the formulation. In contrast, mucosal IgA responses were not detected. In conclusion, it was demonstrated that the use of bioadhesive carriers based on Amioca{\textregistered} starch and poly(acrylic acid) facilitates the induction of a systemic anti-HA antibody response after intranasal vaccination with a whole virus influenza vaccine.",

keywords = "Muco-adhesion, Nasal delivery, Nasal vaccination, Powder formulation",

author = "D. Coucke and M. Schotsaert and C. Libert and E. Pringels and C. Vervaet and P. Foreman and X. Saelens and Remon, {J. P.}",

note = "Funding Information: Mr. D. Tensy is acknowledged for his excellent technical assistance during the animal experiments. We also thank Dr. John Clements (Department of Microbiology and Immunology, Tulane University Medical Center, New Orleans, LA) who generously provided the LTR192G enterotoxin preparation. We are grateful to Dr. Amin Bredan for critical reading of the manuscript. Michael Schotsaert is supported by a grant of the Special Research Fund (BOF, Bijzonder Onderzoeksfonds, B/05930/01) of Ghent University.",

year = "2009",

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doi = "10.1016/j.vaccine.2008.12.013",

language = "English",

volume = "27",

pages = "1279--1286",

journal = "Vaccine",

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T1 - Spray-dried powders of starch and crosslinked poly(acrylic acid) as carriers for nasal delivery of inactivated influenza vaccine

AU - Coucke, D.

AU - Schotsaert, M.

AU - Libert, C.

AU - Pringels, E.

AU - Vervaet, C.

AU - Foreman, P.

AU - Saelens, X.

AU - Remon, J. P.

N1 - Funding Information: Mr. D. Tensy is acknowledged for his excellent technical assistance during the animal experiments. We also thank Dr. John Clements (Department of Microbiology and Immunology, Tulane University Medical Center, New Orleans, LA) who generously provided the LTR192G enterotoxin preparation. We are grateful to Dr. Amin Bredan for critical reading of the manuscript. Michael Schotsaert is supported by a grant of the Special Research Fund (BOF, Bijzonder Onderzoeksfonds, B/05930/01) of Ghent University.

PY - 2009/2/18

Y1 - 2009/2/18

N2 - Mucosal vaccination has several advantages over parenteral vaccination. In this study, viscosity-enhancing mucosal delivery systems for the induction of an adaptive immune response against viral antigen were investigated. Powder formulations based on spray-dried mixtures of starch (Amioca®)/poly(acrylic acid) (Carbopol® 974P) in different ratios were used as carriers of the viral antigen. A comparison of these formulations for intranasal delivery of heat-inactivated influenza virus combined with LTR192G adjuvant was made in vivo in a rabbit model. Individual rabbit sera were tested for seroconversion against hemagglutinin (HA), the major surface antigen of influenza. The powder vaccine formulations were able to induce systemic anti-HA IgG responses. The presence of Carbopol® 974P improved the kinetics of the immune responses and the level of IgG titers in a dose-dependent way which was correlated with moderately irritating capacities of the formulation. In contrast, mucosal IgA responses were not detected. In conclusion, it was demonstrated that the use of bioadhesive carriers based on Amioca® starch and poly(acrylic acid) facilitates the induction of a systemic anti-HA antibody response after intranasal vaccination with a whole virus influenza vaccine.

AB - Mucosal vaccination has several advantages over parenteral vaccination. In this study, viscosity-enhancing mucosal delivery systems for the induction of an adaptive immune response against viral antigen were investigated. Powder formulations based on spray-dried mixtures of starch (Amioca®)/poly(acrylic acid) (Carbopol® 974P) in different ratios were used as carriers of the viral antigen. A comparison of these formulations for intranasal delivery of heat-inactivated influenza virus combined with LTR192G adjuvant was made in vivo in a rabbit model. Individual rabbit sera were tested for seroconversion against hemagglutinin (HA), the major surface antigen of influenza. The powder vaccine formulations were able to induce systemic anti-HA IgG responses. The presence of Carbopol® 974P improved the kinetics of the immune responses and the level of IgG titers in a dose-dependent way which was correlated with moderately irritating capacities of the formulation. In contrast, mucosal IgA responses were not detected. In conclusion, it was demonstrated that the use of bioadhesive carriers based on Amioca® starch and poly(acrylic acid) facilitates the induction of a systemic anti-HA antibody response after intranasal vaccination with a whole virus influenza vaccine.

KW - Muco-adhesion

KW - Nasal delivery

KW - Nasal vaccination

KW - Powder formulation

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DO - 10.1016/j.vaccine.2008.12.013

M3 - Article

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AN - SCOPUS:58749113606

SN - 0264-410X

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EP - 1286

JO - Vaccine

JF - Vaccine

IS - 8

ER -

Spray-dried powders of starch and crosslinked poly(acrylic acid) as carriers for nasal delivery of inactivated influenza vaccine

Abstract

Keywords

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