Spiroimidazolidinone NPC1L1 inhibitors. Part 2: Structure-activity studies and in vivo efficacy

  • Kobporn L. Howell
  • , Robert J. Devita
  • , Margarita Garcia-Calvo
  • , Roger D. Meurer
  • , Jeanmarie Lisnock
  • , Herbert G. Bull
  • , Daniel R. McMasters
  • , Margaret E. McCann
  • , Sander G. Mills

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Ezetimibe (Zetia®), a cholesterol-absorption inhibitor (CAI) approved by the FDA for the treatment of hypercholesterolemia, is believed to target the intestine protein Niemann-Pick C1-Like 1 (NPC1L1) or its pathway. A spiroimidazolidinone NPC1L1 inhibitor identified by virtual screening showed moderate binding activity but was not efficacious in an in vivo rodent model of cholesterol absorption. Synthesis of analogs established the structure-activity relationships for binding activity, and resulted in compounds with in vivo efficacy, including 24, which inhibited plasma cholesterol absorption by 67% in the mouse, thereby providing proof-of-concept that non-β-lactams can be effective CAIs.

Original languageEnglish
Pages (from-to)6929-6932
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume20
Issue number23
DOIs
StatePublished - 1 Dec 2010
Externally publishedYes

Keywords

  • Cholesterol-absorption inhibitor
  • Ezetimibe
  • Hypercholesterolemia
  • Niemann-Pick C1-Like 1 protein
  • Spiroimidazolidinone

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