Abstract
Myelin inhibitors including MAG are major impediments to CNS regeneration. However CNS axons of DRGs regenerate if the peripheral branch of these neurons is lesioned first. We show that 1 day post-peripheral-lesion DRG-cAMP levels triple and MAG/myelin no longer inhibit growth an effect that is PKA dependent. By 1 week post-lesion DRG-cAMP returns to control but growth on MAG/myelin improves and is now PKA independent. Inhibiting PKA in vivo blocks the post-lesion growth on MAG/myelin at 1 day and attenuates it at 1 week. Alone injection of db-cAMP into the DRG mimics completely a conditioning lesion as DRGs grow on MAG/myelin initially in a PKA-dependent manner that becomes PKA independent. Importantly DRG injection of db-cAMP results in extensive regeneration of dorsal column axons lesioned 1 week later. These results may be relevant to developing therapies for spinal cord injury.
Original language | English |
---|---|
Pages (from-to) | 895-903 |
Number of pages | 9 |
Journal | Neuron |
Volume | 34 |
Issue number | 6 |
DOIs | |
State | Published - 13 Jun 2002 |
Externally published | Yes |