Spectrophotometric Assay for Hypochlorite/Hypochlorous Acid Using Tris(2-carboxyethyl)phosphine

Joan Han; Teh Ching Chu; Grace Han; John Browne; Irene Brown; Peter Han

doi:10.1006/mchj.1997.1542

Spectrophotometric Assay for Hypochlorite/Hypochlorous Acid Using Tris(2-carboxyethyl)phosphine

Joan Han, Teh Ching Chu, Grace Han, John Browne, Irene Brown, Peter Han

Research output: Contribution to journal › Article › peer-review

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Abstract

A simple spectrophotometric method for quantitative determination of hypochlorite (OCl^-) or hypochlorous acid (HOCl) is described. The OCl^- or HOCl sample is first incubated with an excess amount of tris(2-carboxyethyl)phosphine (TCEP). The concentration of the residual TCEP is then measured as the amount of 2-nitro-5-thiobenzoate produced after reaction with 5,5′-dithiobis(2-nitrobenzoic acid). The concentration of OCl^- or HOCl is equivalent to the amount of decrease in the concentration of TCEP because one molecule of TCEP is rapidly and irreversibly oxidized to TCEP oxide by one molecule of OCl^- or HOCl. This method is more sensitive and convenient than the standard procedure of NaOCl assay which involves reaction with potassium iodide followed by titration of the liberated triiodide with thiosulfate.

Original language	English
Pages (from-to)	218-224
Number of pages	7
Journal	Microchemical Journal
Volume	58
Issue number	2
DOIs	https://doi.org/10.1006/mchj.1997.1542
State	Published - Feb 1998
Externally published	Yes

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title = "Spectrophotometric Assay for Hypochlorite/Hypochlorous Acid Using Tris(2-carboxyethyl)phosphine",

abstract = "A simple spectrophotometric method for quantitative determination of hypochlorite (OCl-) or hypochlorous acid (HOCl) is described. The OCl- or HOCl sample is first incubated with an excess amount of tris(2-carboxyethyl)phosphine (TCEP). The concentration of the residual TCEP is then measured as the amount of 2-nitro-5-thiobenzoate produced after reaction with 5,5′-dithiobis(2-nitrobenzoic acid). The concentration of OCl- or HOCl is equivalent to the amount of decrease in the concentration of TCEP because one molecule of TCEP is rapidly and irreversibly oxidized to TCEP oxide by one molecule of OCl- or HOCl. This method is more sensitive and convenient than the standard procedure of NaOCl assay which involves reaction with potassium iodide followed by titration of the liberated triiodide with thiosulfate.",

author = "Joan Han and Chu, {Teh Ching} and Grace Han and John Browne and Irene Brown and Peter Han",

note = "Funding Information: This work was supported by NIH Grants SO6GM45199, DRR-RCMIRR03062, and GM08247.",

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T1 - Spectrophotometric Assay for Hypochlorite/Hypochlorous Acid Using Tris(2-carboxyethyl)phosphine

AU - Han, Joan

AU - Chu, Teh Ching

AU - Han, Grace

AU - Browne, John

AU - Brown, Irene

AU - Han, Peter

N1 - Funding Information: This work was supported by NIH Grants SO6GM45199, DRR-RCMIRR03062, and GM08247.

PY - 1998/2

Y1 - 1998/2

N2 - A simple spectrophotometric method for quantitative determination of hypochlorite (OCl-) or hypochlorous acid (HOCl) is described. The OCl- or HOCl sample is first incubated with an excess amount of tris(2-carboxyethyl)phosphine (TCEP). The concentration of the residual TCEP is then measured as the amount of 2-nitro-5-thiobenzoate produced after reaction with 5,5′-dithiobis(2-nitrobenzoic acid). The concentration of OCl- or HOCl is equivalent to the amount of decrease in the concentration of TCEP because one molecule of TCEP is rapidly and irreversibly oxidized to TCEP oxide by one molecule of OCl- or HOCl. This method is more sensitive and convenient than the standard procedure of NaOCl assay which involves reaction with potassium iodide followed by titration of the liberated triiodide with thiosulfate.

AB - A simple spectrophotometric method for quantitative determination of hypochlorite (OCl-) or hypochlorous acid (HOCl) is described. The OCl- or HOCl sample is first incubated with an excess amount of tris(2-carboxyethyl)phosphine (TCEP). The concentration of the residual TCEP is then measured as the amount of 2-nitro-5-thiobenzoate produced after reaction with 5,5′-dithiobis(2-nitrobenzoic acid). The concentration of OCl- or HOCl is equivalent to the amount of decrease in the concentration of TCEP because one molecule of TCEP is rapidly and irreversibly oxidized to TCEP oxide by one molecule of OCl- or HOCl. This method is more sensitive and convenient than the standard procedure of NaOCl assay which involves reaction with potassium iodide followed by titration of the liberated triiodide with thiosulfate.

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DO - 10.1006/mchj.1997.1542

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EP - 224

JO - Microchemical Journal

JF - Microchemical Journal

IS - 2

ER -

Spectrophotometric Assay for Hypochlorite/Hypochlorous Acid Using Tris(2-carboxyethyl)phosphine

Abstract

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