PURPOSE. To test whether the enhanced depth imaging (EDI) modality improves anterior and posterior lamina cribrosa surface (ALCS and PLCS) visibility compared with conventional spectral-domain optical coherence tomography (SD-OCT). METHODS. Conventional and EDI SD-OCT scans were obtained 30 minutes after IOP was manometrically lowered to 10 mm Hg in both eyes of 14 nonhuman primates (NHPs) with unilateral experimental glaucoma (EG). Thirteen horizontal and seven vertical radial B-scans of each SD-OCT data set were delineated by one operator masked to image type. Delineated ALCS and PLCS points were projected to 1 of 100 equal-sized subregions of the neural canal opening (NCO) reference plane, and the number of delineated subregions (≥2 points) was counted. Poisson regression was used to analyze the effects of image type, treatment, and quadrant. Two additional delineations were performed for three NHPs to compare reproducibility. RESULTS. EDI increased the number of subregions delineated for both the ALCS (by 28%; P < 0.0001) and PLCS (by 225%; P <0.0001). EDI improvement in ALCS visibility was significant in the superior quadrant only and was not different in EG versus control eyes, whereas EDI improvement in PLCS visibility was significant in all four quadrants (P ≥0.005) and greater in EG eyes (P ≥0.001), nasally and temporally. Intradelineator reproducibility was not different between image types. EDI and standard ONH parameter values were similar except for PLCS depth which was deeper in the EDI data sets (P ≥ 0.0002). CONCLUSIONS. ALCS and PLCS visibility within control and EG NHP ONHs increased in EDI compared to conventional SDOCT data sets. Further study of EDI effects on PLCS parameterization is required.