Specificity of activation by phosphoinositides determines lipid regulation of Kir channels

Tibor Rohács, Coeli M.B. Lopes, Taihao Jin, Pavan P. Ramdya, Zoltán Molnár, Diomedes E. Logothetis

Research output: Contribution to journalArticlepeer-review

185 Scopus citations

Abstract

Phosphoinositides are critical regulators of ion channel and transporter activity. Defects in interactions of inwardly rectifying potassium (Kir) channels with phosphoinositides lead to disease. ATP-sensitive K+ channels (KATP) are unique among Kir channels in that they serve as metabolic sensors, inhibited by ATP while stimulated by long-chain (LC) acyl-CoA. Here we show that KATP are the least specific Kir channels in their activation by phosphoinositides and we demonstrate that LC acyl-CoA activation of these channels depends on their low phosphoinositide specificity. We provide a systematic characterization of phosphoinositide specificity of the entire Kir channel family expressed in Xenopus oocytes and identify molecular determinants of such specificity. We show that mutations in the Kir2.1 channel decreasing phosphoinositide specificity allow activation by LC acyl-CoA. Our data demonstrate that differences in phosphoinositide specificity determine the modulation of Kir channel activity by distinct regulatory lipids.

Original languageEnglish
Pages (from-to)745-750
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number2
DOIs
StatePublished - 21 Jan 2003
Externally publishedYes

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