Specific receptor for hydrazine: Mapping the: In situ release of hydrazine in live cells and in an in vitro enzymatic assay

Firoj Ali; Anila H. A; Nandaraj Taye; Devraj G. Mogare; Samit Chattopadhyay; Amitava Das

doi:10.1039/c6cc01787h

Specific receptor for hydrazine: Mapping the: In situ release of hydrazine in live cells and in an in vitro enzymatic assay

Firoj Ali, Anila H. A, Nandaraj Taye, Devraj G. Mogare, Samit Chattopadhyay, Amitava Das

Research output: Contribution to journal › Article › peer-review

69 Scopus citations

Abstract

We report a new chemodosimetric reagent capable of detecting hydrazine in the presence of several other competing amine derivatives and ionic analytes of biological relevance. This reagent has been utilized for real time monitoring of in situ N₂H₄ release during the metabolism of a crucial tuberculosis drug, isoniazid, in live HepG2 cells. The fluorescence response of the reagent based on its specific reaction with N₂H₄ is used for developing an in vitro assay for aminoacylase-1.

Original language	English
Pages (from-to)	6166-6169
Number of pages	4
Journal	Chemical Communications
Volume	52
Issue number	36
DOIs	https://doi.org/10.1039/c6cc01787h
State	Published - 2016
Externally published	Yes

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abstract = "We report a new chemodosimetric reagent capable of detecting hydrazine in the presence of several other competing amine derivatives and ionic analytes of biological relevance. This reagent has been utilized for real time monitoring of in situ N2H4 release during the metabolism of a crucial tuberculosis drug, isoniazid, in live HepG2 cells. The fluorescence response of the reagent based on its specific reaction with N2H4 is used for developing an in vitro assay for aminoacylase-1.",

author = "Firoj Ali and A, {Anila H.} and Nandaraj Taye and Mogare, {Devraj G.} and Samit Chattopadhyay and Amitava Das",

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AU - Ali, Firoj

AU - A, Anila H.

AU - Taye, Nandaraj

AU - Mogare, Devraj G.

AU - Chattopadhyay, Samit

AU - Das, Amitava

PY - 2016

Y1 - 2016

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AB - We report a new chemodosimetric reagent capable of detecting hydrazine in the presence of several other competing amine derivatives and ionic analytes of biological relevance. This reagent has been utilized for real time monitoring of in situ N2H4 release during the metabolism of a crucial tuberculosis drug, isoniazid, in live HepG2 cells. The fluorescence response of the reagent based on its specific reaction with N2H4 is used for developing an in vitro assay for aminoacylase-1.

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JO - Chemical Communications

JF - Chemical Communications

IS - 36

ER -

Specific receptor for hydrazine: Mapping the: In situ release of hydrazine in live cells and in an in vitro enzymatic assay

Abstract

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