Spatial regulation of RhoC activity defines protrusion formation in migrating cells

Jose Javier Bravo-Cordero, Ved P. Sharma, Minna Roh-Johnson, Xiaoming Chen, Robert Eddy, John Condeelis, Louis Hodgson

Research output: Contribution to journalArticlepeer-review

53 Scopus citations


Protrusion formation is the first step that precedes cell movement of motile cells. Spatial control of actin polymerization is necessary to achieve directional protrusion during cell migration. Here we show that the spatial coordinators p190RhoGEF and p190RhoGAP regulate actin polymerization during leading edge protrusions by regulating the actin barbed end distribution and amplitude. The distribution of RhoC activity and proper balance of cofilin activation achieved by p190RhoGEF and p190RhoGAP determines the direction of final protrusive activity. These findings provide a new insight into the dynamic plasticity in the amplitude and distribution of barbed ends, which can be modulated by fine-tuning RhoC activity by upstream GEFs and GAPs for directed cell motility.

Original languageEnglish
Pages (from-to)3356-3369
Number of pages14
JournalJournal of Cell Science
Issue number15
StatePublished - 2013
Externally publishedYes


  • Actin cytoskeleton
  • Chemotaxis
  • Directional protrusion
  • RhoC GTPase
  • p190RhoGAP
  • p190RhoGEF


Dive into the research topics of 'Spatial regulation of RhoC activity defines protrusion formation in migrating cells'. Together they form a unique fingerprint.

Cite this