TY - JOUR
T1 - Sox2 + adult stem and progenitor cells are important for tissue regeneration and survival of mice
AU - Arnold, Katrin
AU - Sarkar, Abby
AU - Yram, Mary Anna
AU - Polo, Jose M.
AU - Bronson, Rod
AU - Sengupta, Sumitra
AU - Seandel, Marco
AU - Geijsen, Niels
AU - Hochedlinger, Konrad
N1 - Funding Information:
We thank Laura Prickett-Rice and Kat Folz-Donahoe for assistance with FACS, and Markus Schober, Nimet Maherali, and Matthias Stadtfeld for helpful discussions and critical reading of the manuscript. K.A. and K.H. were supported by a Seed Grant from the Harvard Stem Cell Institute. Additional support to K.H. was from the NIH Director's Innovator Award R01HD058013-01A1, the Kimmel and V Foundations for Cancer Research, and MGH startup funds.
PY - 2011/10/4
Y1 - 2011/10/4
N2 - The transcription factor Sox2 maintains the pluripotency of early embryonic cells and regulates the formation of several epithelia during fetal development. Whether Sox2 continues to play a role in adult tissues remains largely unknown. We show here that Sox2 marks adult cells in several epithelial tissues where its expression has not previously been characterized, including the stomach, cervix, anus, testes, lens, and multiple glands. Genetic lineage tracing and transplantation experiments demonstrate that Sox2-expressing cells continuously give rise to mature cell types within these tissues, documenting their self-renewal and differentiation potentials. Consistent with these findings, ablation of Sox2 + cells in mice results in a disruption of epithelial tissue homeostasis and lethality. Developmental fate mapping reveals that Sox2 + adult stem cells originate from fetal Sox2 + tissue progenitors. Thus, our results identify Sox2 expression in numerous adult endodermal and ectodermal stem cell compartments, which are critical for normal tissue regeneration and survival.
AB - The transcription factor Sox2 maintains the pluripotency of early embryonic cells and regulates the formation of several epithelia during fetal development. Whether Sox2 continues to play a role in adult tissues remains largely unknown. We show here that Sox2 marks adult cells in several epithelial tissues where its expression has not previously been characterized, including the stomach, cervix, anus, testes, lens, and multiple glands. Genetic lineage tracing and transplantation experiments demonstrate that Sox2-expressing cells continuously give rise to mature cell types within these tissues, documenting their self-renewal and differentiation potentials. Consistent with these findings, ablation of Sox2 + cells in mice results in a disruption of epithelial tissue homeostasis and lethality. Developmental fate mapping reveals that Sox2 + adult stem cells originate from fetal Sox2 + tissue progenitors. Thus, our results identify Sox2 expression in numerous adult endodermal and ectodermal stem cell compartments, which are critical for normal tissue regeneration and survival.
UR - http://www.scopus.com/inward/record.url?scp=80053914464&partnerID=8YFLogxK
U2 - 10.1016/j.stem.2011.09.001
DO - 10.1016/j.stem.2011.09.001
M3 - Article
C2 - 21982232
AN - SCOPUS:80053914464
SN - 1934-5909
VL - 9
SP - 317
EP - 329
JO - Cell Stem Cell
JF - Cell Stem Cell
IS - 4
ER -