Sovateltide (IRL-1620) affects neuronal progenitors and prevents cerebral tissue damage after ischemic stroke

Amaresh K. Ranjan, Seema Briyal, Divya Khandekar, Anil Gulati

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Stimulation of endothelin B receptors by its agonist IRL-1620 (INN, sovateltide) provides neuroprotection and neurological and motor function improvement following cerebral ischemia. We investigated the effect of sovateltide on stem and progenitor cells mediated neural regeneration and its effect on the cerebral tissue repair and restoration of neurological and motor function. Sovateltide (5 μg/kg) was injected intravenously in permanent middle cerebral artery occluded (MCAO) rats at 4, 6, and 8 h at days 0, 3, and 6. Neurological and motor function tests were carried out pre-MCAO and at day 7 post-MCAO. At day 7, significantly reduced expression of neuronal differentiation markers HuC/HuD and NeuroD1 was seen in MCAO + vehicle than sham rats. Sovateltide treatment upregulated HuC/HuD and NeuroD1 compared to MCAO + vehicle and their expression was similar to sham. Expression of stem cell markers Oct 4 and Sox 2 was similar in rats of all of the groups. Significantly reduced infarct volume and DNA damage with recovery of neurological and motor function was observed in sovateltide-treated MCAO rats. These results indicate that sovateltide initiates a regenerative response by promoting differentiation of neuronal progenitors and maintaining stem cells in an equilibrium following cerebral ischemic stroke.

Original languageEnglish
Pages (from-to)659-666
Number of pages8
JournalCanadian Journal of Physiology and Pharmacology
Volume98
Issue number9
DOIs
StatePublished - 2020
Externally publishedYes

Keywords

  • Endothelin
  • Endothelin receptors
  • IRL-1620
  • Ischemia
  • Neuronal progenitors
  • Regeneration
  • Stroke

Fingerprint

Dive into the research topics of 'Sovateltide (IRL-1620) affects neuronal progenitors and prevents cerebral tissue damage after ischemic stroke'. Together they form a unique fingerprint.

Cite this