Soluble circulating Fcγ receptors in human serum. A new ELISA assay for specific and quantitative detection

David Khayat; Christine Geffrier; Serena Yoon; Eileen Scigliano; Claude Soubrane; Marise Weil; Jay C. Unkeless; Claude Jacquillat

doi:10.1016/0022-1759(87)90194-3

Soluble circulating Fcγ receptors in human serum. A new ELISA assay for specific and quantitative detection

David Khayat
, Christine Geffrier
, Serena Yoon
, Eileen Scigliano
, Claude Soubrane
, Marise Weil
, Jay C. Unkeless
, Claude Jacquillat

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

We have developed a simple, rapid, and sensitive enzyme-linked immunoadsorbent assay (ELISA) to measure soluble cell-free human Fcγ receptor (FcγR) in serum. This assay is based on the use of two monoclonal antibodies directed against different epitopes expressed on the same low avidity human FcγR (CD16), which is present on polymorphonuclear leukocytes, macrophages and NK cells. This sandwich ELISA, which can measure 2 nM concentration of FcγR, has enabled us to demonstrate the presence and to measure the level of soluble cell-free human FcγR (CfH-FcγR) in normal human serum.

Original language	English
Pages (from-to)	235-241
Number of pages	7
Journal	Journal of Immunological Methods
Volume	100
Issue number	1-2
DOIs	https://doi.org/10.1016/0022-1759(87)90194-3
State	Published - 26 Jun 1987

Keywords

CD16
ELISA
Fcγ receptor
Immunoglobulin binding factor
Monoclonal antibody
Serum
Soluble receptor
human

Access to Document

10.1016/0022-1759(87)90194-3

Cite this

@article{af0b434999834074a90a799322d4116e,

title = "Soluble circulating Fcγ receptors in human serum. A new ELISA assay for specific and quantitative detection",

abstract = "We have developed a simple, rapid, and sensitive enzyme-linked immunoadsorbent assay (ELISA) to measure soluble cell-free human Fcγ receptor (FcγR) in serum. This assay is based on the use of two monoclonal antibodies directed against different epitopes expressed on the same low avidity human FcγR (CD16), which is present on polymorphonuclear leukocytes, macrophages and NK cells. This sandwich ELISA, which can measure 2 nM concentration of FcγR, has enabled us to demonstrate the presence and to measure the level of soluble cell-free human FcγR (CfH-FcγR) in normal human serum.",

keywords = "CD16, ELISA, Fcγ receptor, Immunoglobulin binding factor, Monoclonal antibody, Serum, Soluble receptor, human",

author = "David Khayat and Christine Geffrier and Serena Yoon and Eileen Scigliano and Claude Soubrane and Marise Weil and Unkeless, \{Jay C.\} and Claude Jacquillat",

note = "Funding Information: This investigation was supported by: Grant 6399 of the Association pour la Recherche sur le Cancer (ARC), ViUejuif, France; a grant from the Centre de Recherches Appliqudes \textasciitilde{} la Chimioth6rapie (CRAC), Paris, France; a grant from the Fonds de Bourses de Recherche Scien-tifique et Technique de l'Organisation du Trait6 de l'Atlantique Nord (O.T.A.N.), Paris, France; a grant from the Fondation pour la Recherche Medicale, Paris, France; and by the Public Health Service Grant AI-24322 (J.C.U.). We thank Bernard Colin, Denise Cooper, Brigitte Cedreau and Sophie Visonneau for their technical assistance. We are most grateful to Dr. Victoria Freedman for her help in preparing this manuscript, and to Dr. Dan Serban for his continued support.",

year = "1987",

month = jun,

day = "26",

doi = "10.1016/0022-1759(87)90194-3",

language = "English",

volume = "100",

pages = "235--241",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier B.V.",

number = "1-2",

}

TY - JOUR

T1 - Soluble circulating Fcγ receptors in human serum. A new ELISA assay for specific and quantitative detection

AU - Khayat, David

AU - Geffrier, Christine

AU - Yoon, Serena

AU - Scigliano, Eileen

AU - Soubrane, Claude

AU - Weil, Marise

AU - Unkeless, Jay C.

AU - Jacquillat, Claude

N1 - Funding Information: This investigation was supported by: Grant 6399 of the Association pour la Recherche sur le Cancer (ARC), ViUejuif, France; a grant from the Centre de Recherches Appliqudes ~ la Chimioth6rapie (CRAC), Paris, France; a grant from the Fonds de Bourses de Recherche Scien-tifique et Technique de l'Organisation du Trait6 de l'Atlantique Nord (O.T.A.N.), Paris, France; a grant from the Fondation pour la Recherche Medicale, Paris, France; and by the Public Health Service Grant AI-24322 (J.C.U.). We thank Bernard Colin, Denise Cooper, Brigitte Cedreau and Sophie Visonneau for their technical assistance. We are most grateful to Dr. Victoria Freedman for her help in preparing this manuscript, and to Dr. Dan Serban for his continued support.

PY - 1987/6/26

Y1 - 1987/6/26

N2 - We have developed a simple, rapid, and sensitive enzyme-linked immunoadsorbent assay (ELISA) to measure soluble cell-free human Fcγ receptor (FcγR) in serum. This assay is based on the use of two monoclonal antibodies directed against different epitopes expressed on the same low avidity human FcγR (CD16), which is present on polymorphonuclear leukocytes, macrophages and NK cells. This sandwich ELISA, which can measure 2 nM concentration of FcγR, has enabled us to demonstrate the presence and to measure the level of soluble cell-free human FcγR (CfH-FcγR) in normal human serum.

AB - We have developed a simple, rapid, and sensitive enzyme-linked immunoadsorbent assay (ELISA) to measure soluble cell-free human Fcγ receptor (FcγR) in serum. This assay is based on the use of two monoclonal antibodies directed against different epitopes expressed on the same low avidity human FcγR (CD16), which is present on polymorphonuclear leukocytes, macrophages and NK cells. This sandwich ELISA, which can measure 2 nM concentration of FcγR, has enabled us to demonstrate the presence and to measure the level of soluble cell-free human FcγR (CfH-FcγR) in normal human serum.

KW - CD16

KW - ELISA

KW - Fcγ receptor

KW - Immunoglobulin binding factor

KW - Monoclonal antibody

KW - Serum

KW - Soluble receptor

KW - human

UR - https://www.scopus.com/pages/publications/0023197525

U2 - 10.1016/0022-1759(87)90194-3

DO - 10.1016/0022-1759(87)90194-3

M3 - Article

C2 - 2955053

AN - SCOPUS:0023197525

SN - 0022-1759

VL - 100

SP - 235

EP - 241

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1-2

ER -

Soluble circulating Fcγ receptors in human serum. A new ELISA assay for specific and quantitative detection

Abstract

Keywords

Access to Document

Fingerprint

Cite this