Soluble circulating Fcγ receptors in human serum. A new ELISA assay for specific and quantitative detection

David Khayat, Christine Geffrier, Serena Yoon, Eileen Scigliano, Claude Soubrane, Marise Weil, Jay C. Unkeless, Claude Jacquillat

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

We have developed a simple, rapid, and sensitive enzyme-linked immunoadsorbent assay (ELISA) to measure soluble cell-free human Fcγ receptor (FcγR) in serum. This assay is based on the use of two monoclonal antibodies directed against different epitopes expressed on the same low avidity human FcγR (CD16), which is present on polymorphonuclear leukocytes, macrophages and NK cells. This sandwich ELISA, which can measure 2 nM concentration of FcγR, has enabled us to demonstrate the presence and to measure the level of soluble cell-free human FcγR (CfH-FcγR) in normal human serum.

Original languageEnglish
Pages (from-to)235-241
Number of pages7
JournalJournal of Immunological Methods
Volume100
Issue number1-2
DOIs
StatePublished - 26 Jun 1987

Keywords

  • CD16
  • ELISA
  • Fcγ receptor
  • Immunoglobulin binding factor
  • Monoclonal antibody
  • Serum
  • Soluble receptor
  • human

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