Abstract
The Epstein-Barr virus (EBV)-determined nuclear antigen (EBNA) was solubilized from isolated nuclei of two EBV-transformed cell lines, Raji and AW-Ramos, by high-salt treatment. Its DNA-binding properties were studied by DNA-cellulose chromatography and a 51Cr release complement fixation assay. EBNA binds to both double-stranded and single-stranded calf thymus DNA, showing a higher affinity to double-stranded DNA. There was no detectable difference in the DNA binding of EBNA prepared from Raji and AW-Ramos cells.
| Original language | English |
|---|---|
| Pages (from-to) | 1-8 |
| Number of pages | 8 |
| Journal | Journal of Virology |
| Volume | 22 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1977 |
| Externally published | Yes |