Solubilisation of Epstein-Barr virus (ebv)-associated nuclear antigen from Raji cells and chromatin by treatment with various molarities of NaCl

T. D.K. Brown; D. Rickwood; A. J. Macgillivray; G. Klein

doi:10.1016/S0304-3835(77)95187-4

Solubilisation of Epstein-Barr virus (ebv)-associated nuclear antigen from Raji cells and chromatin by treatment with various molarities of NaCl

T. D.K. Brown, D. Rickwood, A. J. Macgillivray, G. Klein

Research output: Contribution to journal › Article › peer-review

Abstract

The solubilisation of Epstein-Barr virus (EBV)-associated nuclear antigen (EBNA) by treatment with various molarities of NaCl was investigated using the ¹²⁵I-IgG absorption assay. Ninety percent of the antigenic activity detected using the ¹²⁵I-IgG absorption assay was insoluble at 0.15 M NaCl. It could be rendered soluble by treatment with 2.0 M NaCl, but reprecipitated upon return to 0.15 M NaCl. EBNA was partially extracted from Raji chromatin by treatment with 0.35 M NaCl. The efficiency of extraction was increased by homogenisation in 2.0 M NaCl followed by dialysis to 0.35 M NaCl. The data demonstrate the close association of EBNA with Raji chromatin and suggest that it may be a chromatin-associated non-histone protein.

Original language	English
Pages (from-to)	151-156
Number of pages	6
Journal	Cancer Letters
Volume	3
Issue number	C
DOIs	https://doi.org/10.1016/S0304-3835(77)95187-4
State	Published - 1977
Externally published	Yes

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@article{ae31903b53974b6cb2b240f588038022,

title = "Solubilisation of Epstein-Barr virus (ebv)-associated nuclear antigen from Raji cells and chromatin by treatment with various molarities of NaCl",

abstract = "The solubilisation of Epstein-Barr virus (EBV)-associated nuclear antigen (EBNA) by treatment with various molarities of NaCl was investigated using the 125I-IgG absorption assay. Ninety percent of the antigenic activity detected using the 125I-IgG absorption assay was insoluble at 0.15 M NaCl. It could be rendered soluble by treatment with 2.0 M NaCl, but reprecipitated upon return to 0.15 M NaCl. EBNA was partially extracted from Raji chromatin by treatment with 0.35 M NaCl. The efficiency of extraction was increased by homogenisation in 2.0 M NaCl followed by dialysis to 0.35 M NaCl. The data demonstrate the close association of EBNA with Raji chromatin and suggest that it may be a chromatin-associated non-histone protein.",

author = "Brown, \{T. D.K.\} and D. Rickwood and Macgillivray, \{A. J.\} and G. Klein",

note = "Funding Information: This work was supported by contract NO1-33316 within the Virus Cancer Programme of the National Cancer Institute and by a grant from the Swedish Cancer Society. T.D.K. Brown was initially the recipient of a European Exchange Fellowship: administered by the Royal Society and subsequently a research fellow of Cancer Research Institute, Inc., New York. We wish to thank Mona Hansson, Robert McFarlane and Mr. A.C. McKirdy for excellent technical assistance.",

year = "1977",

doi = "10.1016/S0304-3835(77)95187-4",

language = "English",

volume = "3",

pages = "151--156",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "C",

}

TY - JOUR

T1 - Solubilisation of Epstein-Barr virus (ebv)-associated nuclear antigen from Raji cells and chromatin by treatment with various molarities of NaCl

AU - Brown, T. D.K.

AU - Rickwood, D.

AU - Macgillivray, A. J.

AU - Klein, G.

N1 - Funding Information: This work was supported by contract NO1-33316 within the Virus Cancer Programme of the National Cancer Institute and by a grant from the Swedish Cancer Society. T.D.K. Brown was initially the recipient of a European Exchange Fellowship: administered by the Royal Society and subsequently a research fellow of Cancer Research Institute, Inc., New York. We wish to thank Mona Hansson, Robert McFarlane and Mr. A.C. McKirdy for excellent technical assistance.

PY - 1977

Y1 - 1977

N2 - The solubilisation of Epstein-Barr virus (EBV)-associated nuclear antigen (EBNA) by treatment with various molarities of NaCl was investigated using the 125I-IgG absorption assay. Ninety percent of the antigenic activity detected using the 125I-IgG absorption assay was insoluble at 0.15 M NaCl. It could be rendered soluble by treatment with 2.0 M NaCl, but reprecipitated upon return to 0.15 M NaCl. EBNA was partially extracted from Raji chromatin by treatment with 0.35 M NaCl. The efficiency of extraction was increased by homogenisation in 2.0 M NaCl followed by dialysis to 0.35 M NaCl. The data demonstrate the close association of EBNA with Raji chromatin and suggest that it may be a chromatin-associated non-histone protein.

AB - The solubilisation of Epstein-Barr virus (EBV)-associated nuclear antigen (EBNA) by treatment with various molarities of NaCl was investigated using the 125I-IgG absorption assay. Ninety percent of the antigenic activity detected using the 125I-IgG absorption assay was insoluble at 0.15 M NaCl. It could be rendered soluble by treatment with 2.0 M NaCl, but reprecipitated upon return to 0.15 M NaCl. EBNA was partially extracted from Raji chromatin by treatment with 0.35 M NaCl. The efficiency of extraction was increased by homogenisation in 2.0 M NaCl followed by dialysis to 0.35 M NaCl. The data demonstrate the close association of EBNA with Raji chromatin and suggest that it may be a chromatin-associated non-histone protein.

UR - https://www.scopus.com/pages/publications/0017735375

U2 - 10.1016/S0304-3835(77)95187-4

DO - 10.1016/S0304-3835(77)95187-4

M3 - Article

C2 - 198113

AN - SCOPUS:0017735375

SN - 0304-3835

VL - 3

SP - 151

EP - 156

JO - Cancer Letters

JF - Cancer Letters

IS - C

ER -

Solubilisation of Epstein-Barr virus (ebv)-associated nuclear antigen from Raji cells and chromatin by treatment with various molarities of NaCl

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