Solid-phase radioimmunoassay for estimation of elastin peptides in human sera

T. V. Darnule; M. McKee; A. T. Darnule; G. M. Turino; I. Mandl

doi:10.1016/0003-2697(82)90286-X

Solid-phase radioimmunoassay for estimation of elastin peptides in human sera

T. V. Darnule, M. McKee, A. T. Darnule, G. M. Turino, I. Mandl

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

A sensitive, reproducible, and rapid radioimmunoassay was developed to determine clastin peptide content of human serum in nanogram amounts. Significant differences have been determined between normal volunteers, both smokers and nonsmokers, and patients wth chronic obstructive pulmonary disease. The average concentration of clastin peptides in 9 normal nonsmokers was 19.88 ± 10.46 μg/ml serum, indistinguishable from that in 7 normal smokers which was 17.64 ± 8.54 μg/ml. Average concentration of clastin peptides in 17 patients with lung disease was 53.38 ± 22.46 μg/ml, significantly different (P < 0.001) from the healthy control group. The method outlined shows excellent reproducibility on multiple determinations and allows analysis of as many as 100 samples in 6-8 h.

Original language	English
Pages (from-to)	302-307
Number of pages	6
Journal	Analytical Biochemistry
Volume	122
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(82)90286-X
State	Published - 15 May 1982
Externally published	Yes

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@article{0619e947aaea48b1953208d92ce52851,

title = "Solid-phase radioimmunoassay for estimation of elastin peptides in human sera",

abstract = "A sensitive, reproducible, and rapid radioimmunoassay was developed to determine clastin peptide content of human serum in nanogram amounts. Significant differences have been determined between normal volunteers, both smokers and nonsmokers, and patients wth chronic obstructive pulmonary disease. The average concentration of clastin peptides in 9 normal nonsmokers was 19.88 ± 10.46 μg/ml serum, indistinguishable from that in 7 normal smokers which was 17.64 ± 8.54 μg/ml. Average concentration of clastin peptides in 17 patients with lung disease was 53.38 ± 22.46 μg/ml, significantly different (P < 0.001) from the healthy control group. The method outlined shows excellent reproducibility on multiple determinations and allows analysis of as many as 100 samples in 6-8 h.",

author = "Darnule, {T. V.} and M. McKee and Darnule, {A. T.} and Turino, {G. M.} and I. Mandl",

note = "Funding Information: This work was supported in part by USPHS Program Project Grant HL15832 and New York State Health Research Council award HRC 1554. During this work T.V.D. was recipient of a Parker B. Francis Foundation Fellowship in Pulmonary Research and a National Research Service Award (T32-H607018-06) Trainee. The authors gratefully acknowledge the skilled technical assistance of Miss Jane McCarthy.",

year = "1982",

month = may,

day = "15",

doi = "10.1016/0003-2697(82)90286-X",

language = "English",

volume = "122",

pages = "302--307",

journal = "Analytical Biochemistry",

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number = "2",

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TY - JOUR

T1 - Solid-phase radioimmunoassay for estimation of elastin peptides in human sera

AU - Darnule, T. V.

AU - McKee, M.

AU - Darnule, A. T.

AU - Turino, G. M.

AU - Mandl, I.

N1 - Funding Information: This work was supported in part by USPHS Program Project Grant HL15832 and New York State Health Research Council award HRC 1554. During this work T.V.D. was recipient of a Parker B. Francis Foundation Fellowship in Pulmonary Research and a National Research Service Award (T32-H607018-06) Trainee. The authors gratefully acknowledge the skilled technical assistance of Miss Jane McCarthy.

PY - 1982/5/15

Y1 - 1982/5/15

N2 - A sensitive, reproducible, and rapid radioimmunoassay was developed to determine clastin peptide content of human serum in nanogram amounts. Significant differences have been determined between normal volunteers, both smokers and nonsmokers, and patients wth chronic obstructive pulmonary disease. The average concentration of clastin peptides in 9 normal nonsmokers was 19.88 ± 10.46 μg/ml serum, indistinguishable from that in 7 normal smokers which was 17.64 ± 8.54 μg/ml. Average concentration of clastin peptides in 17 patients with lung disease was 53.38 ± 22.46 μg/ml, significantly different (P < 0.001) from the healthy control group. The method outlined shows excellent reproducibility on multiple determinations and allows analysis of as many as 100 samples in 6-8 h.

AB - A sensitive, reproducible, and rapid radioimmunoassay was developed to determine clastin peptide content of human serum in nanogram amounts. Significant differences have been determined between normal volunteers, both smokers and nonsmokers, and patients wth chronic obstructive pulmonary disease. The average concentration of clastin peptides in 9 normal nonsmokers was 19.88 ± 10.46 μg/ml serum, indistinguishable from that in 7 normal smokers which was 17.64 ± 8.54 μg/ml. Average concentration of clastin peptides in 17 patients with lung disease was 53.38 ± 22.46 μg/ml, significantly different (P < 0.001) from the healthy control group. The method outlined shows excellent reproducibility on multiple determinations and allows analysis of as many as 100 samples in 6-8 h.

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U2 - 10.1016/0003-2697(82)90286-X

DO - 10.1016/0003-2697(82)90286-X

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AN - SCOPUS:0020322233

SN - 0003-2697

VL - 122

SP - 302

EP - 307

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Solid-phase radioimmunoassay for estimation of elastin peptides in human sera

Abstract

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