@article{25028e8ff4784c4ab2a37786f4a13a46,
title = "SNAREpins: Minimal machinery for membrane fusion",
abstract = "Recombinant v- and t-SNARE proteins reconstituted into separate lipid bilayer vesicles assemble into SNARE-pins-SNARE complexes linking two membranes. This leads to spontaneous fusion of the docked membranes at physiological temperature. Docked unfused intermediates can accumulate at lower temperatures and can fuse when brought to physiological temperature. A supply of unassembled v- and t-SNAREs is needed for these intermediates to form, but not for the fusion that follows. These data imply that SNARE-pins are the minimal machinery for cellular membrane fusion.",
author = "Thomas Weber and Zemelman, \{Boris V.\} and McNew, \{James A.\} and Benedikt Westermann and Michael Gmachl and Francesco Parlati and S{\"o}llner, \{Thomas H.\} and Rothman, \{James E.\}",
note = "Funding Information: We thank Dr. Heiner Niemann for the expression clone encoding the light chain of botulinum neurotoxin D, Dr. Reinhard Jahn for the hybridoma cell line Cl. 69.1, Dr. Hediye Erdjument-Bromage and the Microchemistry Core Facility of the Memorial Sloan-Kettering Cancer Center for protein sequencing, Nancy Arango and William Eng for technical assistance, and Joshua C. Olesker and Jason Kurtz for help with the manuscript. We would also like to thank Dr. Donald M. Engelman for helpful discussions concerning possible fusion mechanisms. Artwork for Figure 5 and the cover of this issue was prepared by Craig Foster of Foster Medical Communications. Research was supported by the Mathers Charitable Foundation (to J. E. R.); a National Institute of Health (NIH) grant (to J. E. R); and postdoctoral fellowships of the Swiss National Science Foundation (to T. W.), of the European Molecular Biology Organization (to T. W.), of the Deutsche Forschungsgemeinschaft (to B. W.), of the NIH (to B. V. Z. and J. A. M.), of the Max Kade Foundation (to M. G.), and of the Medical Research Council Canada (to F. P.).",
year = "1998",
month = mar,
day = "20",
doi = "10.1016/S0092-8674(00)81404-X",
language = "English",
volume = "92",
pages = "759--772",
journal = "Cell",
issn = "0092-8674",
publisher = "Elsevier B.V.",
number = "6",
}