SNAREpins are functionally resistant to disruption by NSF and αSNAP

Thomas Weber, Francesco Parlati, James A. McNew, Robert J. Johnston, Benedikt Westermann, Thomas H. Söllner, James E. Rothman

Research output: Contribution to journalArticlepeer-review

107 Scopus citations

Abstract

SNARE (SNAP [soluble NSF {N-ethylmaleimide-sensitive fusion protein} attachment protein] receptor) proteins are required for many fusion processes, and recent studies of isolated SNARE proteins reveal that they are inherently capable of fusing lipid bilayers. Cis-SNARE complexes (formed when vesicle SNAREs [v-SNAREs] and target membrane SNAREs [t-SNAREs] combine in the same membrane) are disrupted by the action of the abundant cytoplasmic ATPase NSF, which is necessary to maintain a supply of uncombined v- and t- SNAREs for fusion in cells. Fusion is mediated by these same SNARE proteins, forming trans-SNARE complexes between membranes. This raises an important question: why doesn't NSF disrupt these SNARE complexes as well, preventing fusion from occurring at all? Here, we report several lines of evidence that demonstrate that SNAREpins (trans-SNARE complexes) are in fact functionally resistant to NSF, and they become so at the moment they form and commit to fusion. This elegant design allows fusion to proceed locally in the face of an overall environment that massively favors SNARE disruption.

Original languageEnglish
Pages (from-to)1063-1072
Number of pages10
JournalJournal of Cell Biology
Volume149
Issue number5
DOIs
StatePublished - 29 May 2000

Keywords

  • Liposomes
  • Membrane fusion
  • NSF
  • SNARE
  • αSNAP

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