Smyd2 controls cytoplasmic lysine methylation of Hsp90 and myofilament organization

Laura T. Donlin, Christian Andresen, Steffen Just, Eugene Rudensky, Christopher T. Pappas, Martina Kruger, Erica Y. Jacobs, Andreas Unger, Anke Zieseniss, Marc Werner Dobenecker, Tobias Voelkel, Brian T. Chait, Carol C. Gregorio, Wolfgang Rottbauer, Alexander Tarakhovsky, Wolfgang A. Linke

Research output: Contribution to journalArticlepeer-review

132 Scopus citations


Protein lysine methylation is one of the most widespread post-translational modifications in the nuclei of eukaryotic cells. Methylated lysines on histones and nonhistone proteins promote the formation of protein complexes that control gene expression and DNA replication and repair. In the cytoplasm, however, the role of lysine methylation in protein complex formation is not well established. Here we report that the cytoplasmic protein chaperone Hsp90 is methylated by the lysine methyltransferase Smyd2 in various cell types. In muscle, Hsp90 methylation contributes to the formation of a protein complex containing Smyd2, Hsp90, and the sarcomeric protein titin. Deficiency in Smyd2 results in the loss of Hsp90 methylation, impaired titin stability, and altered muscle function. Collectively, our data reveal a cytoplasmic protein network that employs lysine methylation for the maintenance and function of skeletal muscle.

Original languageEnglish
Pages (from-to)114-119
Number of pages6
JournalGenes and Development
Issue number2
StatePublished - 15 Jan 2012
Externally publishedYes


  • Hsp90
  • I-band
  • Lysine methylation
  • Sarcomere
  • Smyd2
  • Titin


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