Smarca4 Inactivation Promotes LineageSpecific Transformation and Early Metastatic Features in the Lung

Carla P. Concepcion, Sai Ma, Lindsay M. Lafave, Arjun Bhutkar, Manyuan Liu, Lydia P. Deangelo, Jonathan Y. Kim, Isabella Del Priore, Adam J. Schoenfeld, Manon Miller, Vinay K. Kartha, Peter M.K. Westcott, Francisco J. Sánchez-Rivera, Kevin Meli, Manav Gupta, Roderick T. Bronson, Gregory J. Riely, Natasha Rekhtman, Charles M. Rudin, Carla F. KimAviv Regev, Jason D. Buenrostro, Tyler Jacks

Research output: Contribution to journalArticlepeer-review

49 Scopus citations


SMARCA4/BRG1 encodes for one of two mutually exclusive ATPases present in mammalian SWI/SNF chromatin remodeling complexes and is frequently mutated in human lung adenocarcinoma. However, the functional consequences of SMARCA4 mutation on tumor initiation, progression, and chromatin regulation in lung cancer remain poorly understood. Here, we demonstrate that loss of Smarca4 sensitizes club cell secretory protein–positive cells within the lung in a cell type–dependent fashion to malignant transformation and tumor progression, resulting in highly advanced dedifferentiated tumors and increased metastatic incidence. Consistent with these phenotypes, Smarca4-deficient primary tumors lack lung lineage transcription factor activities and resemble a metastatic cell state. Mechanistically, we show that Smarca4 loss impairs the function of all three classes of SWI/SNF complexes, resulting in decreased chromatin accessibility at lung lineage motifs and ultimately accelerating tumor progression. Thus, we propose that the SWI/SNF complex via Smarca4 acts as a gatekeeper for lineage-specific cellular transformation and metastasis during lung cancer evolution. SIGNIFICANCE: We demonstrate cell-type specificity in the tumor-suppressive functions of SMARCA4 in the lung, pointing toward a critical role of the cell-of-origin in driving SWI/SNF-mutant lung adenocarcinoma. We further show the direct effects of SMARCA4 loss on SWI/SNF function and chromatin regulation that cause aggressive malignancy during lung cancer evolution.

Original languageEnglish
Pages (from-to)562-585
Number of pages24
JournalCancer Discovery
Issue number2
StatePublished - 1 Feb 2022
Externally publishedYes


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