Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon

Masayoshi Fukuda, Tomohiro Mizutani, Wakana Mochizuki, Taichi Matsumoto, Kengo Nozaki, Yuriko Sakamaki, Shizuko Ichinose, Yukinori Okada, Toshihiro Tanaka, Mamoru Watanabe, Tetsuya Nakamura

Research output: Contribution to journalArticlepeer-review

106 Scopus citations

Abstract

To develop stem cell therapy for small intestinal (SI) diseases, it is essential to determine whether SI stem cells in culture retain their tissue regeneration capabilities. By using a heterotopic transplantation approach, we show that cultured murine SI epithelial organoids are able to reconstitute self-renewing epithelia in the colon. When stably integrated, the SI-derived grafts show many features unique only to the SI but distinct from the colonic epithelium. Our study provides evidence that cultured adult SI stem cells could be a source for cell therapy of intestinal diseases, maintaining their identity along the gastrointestinal tract through an epithelium-intrinsic mechanism.

Original languageEnglish
Pages (from-to)1752-1757
Number of pages6
JournalGenes and Development
Volume28
Issue number16
DOIs
StatePublished - 15 Aug 2014
Externally publishedYes

Keywords

  • Paneth cell
  • Regenerative medicine
  • Small intestinal stem cell
  • Stem cell niche
  • Transplantation

Fingerprint

Dive into the research topics of 'Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon'. Together they form a unique fingerprint.

Cite this