SM-20 is a novel growth factor-responsive gene regulated during skeletal muscle development and differentiation

Maria C. Moschella, Keon Menzies, Lana Tsao, Mark A. Lieb, Jhumku D. Kohtz, D. Stave Kohtz, Mark B. Taubman

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


SM-20 is a novel, evolutionarily conserved 'early response' gene originally cloned from a rat aortic smooth muscle cell (SMC) cDNA library. SM-20 encodes a cytoplasmic protein, which is induced by platelet-derived growth factor and angiotensin II in cultured SMC and is upregulated in intimal SMC of atherosclerotic plaques and injured arteries. We have now examined SM-20 expression during differentiation of cultured skeletal myoblasts and during skeletal myogenesis in vivo. Low levels of SM-20 mRNA and protein were expressed in proliferating mouse C2C12 myoblasts. Differentiation by serum withdrawal was associated with a marked induction of SM-20 mRNA and the expression of high levels of SM-20 antigen in myotubes. The induction was partially inhibited by blocking differentiation with bFGF or TGFβ. Similar results were obtained with the nonfusing mouse C25 myoblast line, suggesting that SM-20 upregulation is a consequence of biochemical differentiation and is fusion independent. During mouse embryogenesis, SM-20 was first observed at 8.5E in the dermomyotomal cells of the rostral somites. SM-20 expression progressed in a rostral to caudal pattern, with highest levels seen in the muscle primordia and mature muscles. SM-20 thus represents a novel intracellular protein that is regulated during skeletal muscle differentiation and development.

Original languageEnglish
Pages (from-to)59-66
Number of pages8
JournalGene Expression
Issue number1
StatePublished - 1999


  • Antibody staining
  • Mouse embryo
  • Myocyte differentiation
  • Myogenesis
  • SM-20
  • Skeletal muscle
  • Somites


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