TY - JOUR
T1 - Slow-reacting substances, leukotrienes C4 and D4, increase the release of mucus from human airways in vitro
AU - Marom, Z.
AU - Shelhamer, J. H.
AU - Bach, M. K.
AU - Morton, D. R.
AU - Kaliner, M.
PY - 1982
Y1 - 1982
N2 - Allergic pulmonary reactions in vivo lead to airway construction and mucous secretion, whereas in vitro lung anaphylaxis leads to mediator release and increased mucous glycoprotein secretion from cultured human airways. Using quantitation of radiolabeled mucous glycoprotein from cultured airways as a model for mucous release, the effects of two leukotrienes, LTC4 and LTD4, were assessed. Both biosynthetic and synthetic LTC4 and LTD4 produced dose-related increases in mucous production at concentrations of 1 to 1,000 units/ml (20 to 20,000 pg [LTD4] and 24 to 24,000 pg [LTC4l/ml). These enhancing actions were seen in 11 of 11 lung cultures, and were significantly prevented by the specific SRS-A antagonist FPL 55712. Therefore, LTC4 and LTD4, two major components of the allergic mediator SRS-A, are potent mucous secretagogues and may possibly contribute to the mucous secretion observed during allergic reactions in vitro and in vivo.
AB - Allergic pulmonary reactions in vivo lead to airway construction and mucous secretion, whereas in vitro lung anaphylaxis leads to mediator release and increased mucous glycoprotein secretion from cultured human airways. Using quantitation of radiolabeled mucous glycoprotein from cultured airways as a model for mucous release, the effects of two leukotrienes, LTC4 and LTD4, were assessed. Both biosynthetic and synthetic LTC4 and LTD4 produced dose-related increases in mucous production at concentrations of 1 to 1,000 units/ml (20 to 20,000 pg [LTD4] and 24 to 24,000 pg [LTC4l/ml). These enhancing actions were seen in 11 of 11 lung cultures, and were significantly prevented by the specific SRS-A antagonist FPL 55712. Therefore, LTC4 and LTD4, two major components of the allergic mediator SRS-A, are potent mucous secretagogues and may possibly contribute to the mucous secretion observed during allergic reactions in vitro and in vivo.
UR - http://www.scopus.com/inward/record.url?scp=0019971503&partnerID=8YFLogxK
M3 - Article
C2 - 7125334
AN - SCOPUS:0019971503
SN - 0003-0805
VL - 126
SP - 449
EP - 451
JO - American Review of Respiratory Disease
JF - American Review of Respiratory Disease
IS - 3
ER -