Slipped DNA structures within the enhancer region of the moloney murine leukemia virus

Miguel A. Gama Sosa, Jessica C. Hall, Ruth M. Ruprecht

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

We have examined the Sl nuclease sensitivity of supercoiled plasmids harboring the Moloney Murine Leukemia Virus (MoMuLV) long terminal repeat (LTR). S1 sensitivity was found within the LTR enhancer direct repeats. Transformation of E. coli DH5 cells with a construct containing most of the MoMuLV LTR yielded the precise deletion of one direct repeat and loss of S1 sensitivity. The dependence of S1 sensitivity on the presence of both direct repeats, together with the exact excision of one direct repeat by E. coli, suggests the presence of slipped DNA within the enhancer. Such structures may represent targets for effector proteins which mediate vital functions during viral propagation.

Original languageEnglish
Pages (from-to)417-423
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume156
Issue number1
DOIs
StatePublished - 14 Oct 1988
Externally publishedYes

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