SKK4, a novel activator of stress-activated protein kinase-1

Sean Lawler; Ana Cuenda; Michel Goedert; Philip Cohen

doi:10.1016/S0014-5793(97)00990-3

SKK4, a novel activator of stress-activated protein kinase-1

Sean Lawler
, Ana Cuenda
, Michel Goedert
, Philip Cohen

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

A cDNA was cloned and expressed that encodes human stress-activated protein kinase kinase-4 (SKK4), a novel MAP kinase kinase family member whose mRNA is widely expressed in human tissues. SKK4 activated SAPK1/JNK in vitro, but not SAPK2a/p38, SAPK2b/p38β, SAPK3/ERK6 or SAPK4. It appears to be the mammalian homologue of HEP, an activator of SAPK1/JNK in Drosophila. In human epithelial KB cells SKK4 and SKK1/MKK4 (another activator of SAPK1/ JNK) were both activated by stressful stimuli, but only SKK4 was activated by proinflammatory cytokines. The identification of SKK4 explains why the major SAPK1/JNK activator detected in many mammalian cell extracts is chromatographically separable from SKK1/MKK4.

Original language	English
Pages (from-to)	153-158
Number of pages	6
Journal	FEBS Letters
Volume	414
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(97)00990-3
State	Published - 1 Sep 1997
Externally published	Yes

Keywords

Cytokine
JNK
MAP kinase
SAPK
Stress
p38

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10.1016/S0014-5793(97)00990-3

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title = "SKK4, a novel activator of stress-activated protein kinase-1",

abstract = "A cDNA was cloned and expressed that encodes human stress-activated protein kinase kinase-4 (SKK4), a novel MAP kinase kinase family member whose mRNA is widely expressed in human tissues. SKK4 activated SAPK1/JNK in vitro, but not SAPK2a/p38, SAPK2b/p38β, SAPK3/ERK6 or SAPK4. It appears to be the mammalian homologue of HEP, an activator of SAPK1/JNK in Drosophila. In human epithelial KB cells SKK4 and SKK1/MKK4 (another activator of SAPK1/ JNK) were both activated by stressful stimuli, but only SKK4 was activated by proinflammatory cytokines. The identification of SKK4 explains why the major SAPK1/JNK activator detected in many mammalian cell extracts is chromatographically separable from SKK1/MKK4.",

keywords = "Cytokine, JNK, MAP kinase, SAPK, Stress, p38",

author = "Sean Lawler and Ana Cuenda and Michel Goedert and Philip Cohen",

year = "1997",

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doi = "10.1016/S0014-5793(97)00990-3",

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T1 - SKK4, a novel activator of stress-activated protein kinase-1

AU - Lawler, Sean

AU - Cuenda, Ana

AU - Goedert, Michel

AU - Cohen, Philip

PY - 1997/9/1

Y1 - 1997/9/1

N2 - A cDNA was cloned and expressed that encodes human stress-activated protein kinase kinase-4 (SKK4), a novel MAP kinase kinase family member whose mRNA is widely expressed in human tissues. SKK4 activated SAPK1/JNK in vitro, but not SAPK2a/p38, SAPK2b/p38β, SAPK3/ERK6 or SAPK4. It appears to be the mammalian homologue of HEP, an activator of SAPK1/JNK in Drosophila. In human epithelial KB cells SKK4 and SKK1/MKK4 (another activator of SAPK1/ JNK) were both activated by stressful stimuli, but only SKK4 was activated by proinflammatory cytokines. The identification of SKK4 explains why the major SAPK1/JNK activator detected in many mammalian cell extracts is chromatographically separable from SKK1/MKK4.

AB - A cDNA was cloned and expressed that encodes human stress-activated protein kinase kinase-4 (SKK4), a novel MAP kinase kinase family member whose mRNA is widely expressed in human tissues. SKK4 activated SAPK1/JNK in vitro, but not SAPK2a/p38, SAPK2b/p38β, SAPK3/ERK6 or SAPK4. It appears to be the mammalian homologue of HEP, an activator of SAPK1/JNK in Drosophila. In human epithelial KB cells SKK4 and SKK1/MKK4 (another activator of SAPK1/ JNK) were both activated by stressful stimuli, but only SKK4 was activated by proinflammatory cytokines. The identification of SKK4 explains why the major SAPK1/JNK activator detected in many mammalian cell extracts is chromatographically separable from SKK1/MKK4.

KW - Cytokine

KW - JNK

KW - MAP kinase

KW - SAPK

KW - Stress

KW - p38

UR - https://www.scopus.com/pages/publications/0030863983

U2 - 10.1016/S0014-5793(97)00990-3

DO - 10.1016/S0014-5793(97)00990-3

M3 - Article

C2 - 9305750

AN - SCOPUS:0030863983

SN - 0014-5793

VL - 414

SP - 153

EP - 158

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

SKK4, a novel activator of stress-activated protein kinase-1

Abstract

Keywords

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