Site-specific tamoxifen-DNA adduct formation: Lack of correlation with mutational ability in Escherichia coli

Damon A. Lowes, Karen Brown, Robert T. Heydon, Elizabeth A. Martin, Timothy W. Gant

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


We have mapped sites of tamoxifen adduct formation, in the lacI gene using the polymerase STOP assay, following reaction in vitro with α- acetoxytamoxifen and horseradish peroxidase (HRP)/H2O2 activated 4- hydroxytamoxifen. For both compounds, most adduct formation occurred on guanines. However, one adenine, within a run of guanines, generated a strong polymerase STOP site with activated 4-hydroxytamoxifen, and a weaker STOP site with α-acetoxytamoxifen at the same location. In Escherichia coli the lac I gene reacted with 4-hydroxytamoxifen was more likely to be mutated (2 orders of magnitude) than when reacted with α-acetoxytamoxifen, despite the greater DNA adduct formation by α-acetoxytamoxifen. This correlates with the greater predicted ability of activated 4-hydroxytamoxifen adducts to disrupt DNA structure than α-acetoxytamoxifen adducts. For lac I reacted with activated 4-hydroxytamoxifen, a hot spot of base mutation was located in the region of the only adenosine adduct. No mutational hot spots were observed with α-acetoxytamoxifen. Our data clearly shows a lack of correlation between gross adduct number, as assayed by 32P-postlabeling and mutagenic potential. These data indicate the importance of minor adduct formation in mutagenic potential and further that conclusions regarding the mutagenicity of a chemical may not be reliably derived from the gross determination of adduct formation.

Original languageEnglish
Pages (from-to)10989-10996
Number of pages8
Issue number34
StatePublished - 24 Aug 1999
Externally publishedYes


Dive into the research topics of 'Site-specific tamoxifen-DNA adduct formation: Lack of correlation with mutational ability in Escherichia coli'. Together they form a unique fingerprint.

Cite this