Sirt5 is dispensable for Braf V600E -mediated cutaneous melanoma development and growth in vivo

Hyeongsun Moon, Jerry Zhu, Andrew C. White

Research output: Contribution to journalLetterpeer-review

9 Scopus citations

Abstract

Sirt5 is known to functionally regulate mitochondrial proteins by altering posttranslational modifications, including lysine desuccinylation. While roles for Sirt5 as either a tumor promoter or suppressor, or in chemoresistance, have been implicated in other cancers, the function of Sirt5 in cutaneous melanoma has not been well examined. Therefore, to determine whether Sirt5 is necessary for Braf V600E -mediated melanoma formation and/or disease progression, we crossed a genetically engineered murine melanoma model (Tyr Cre ERT 2/+ ; Braf LSL -V600E/+ ; Pten flox/flox ) to Sirt5 −/− knockout animals. In addition, we tested for synergism with a selective BRAF (V600E) inhibitor in Sirt5 −/− mouse melanoma cells. Taken together, this report demonstrates that, in these models, Sirt5 is dispensable for Braf V600E -mediated cutaneous melanoma formation and growth in vivo, and does not improve sensitivity to a selective BRAF inhibitor.

Original languageEnglish
Pages (from-to)83-85
Number of pages3
JournalExperimental Dermatology
Volume28
Issue number1
DOIs
StatePublished - Jan 2019
Externally publishedYes

Keywords

  • Braf
  • Sirt5
  • cutaneous melanoma
  • melanocyte stem cells

Fingerprint

Dive into the research topics of 'Sirt5 is dispensable for Braf V600E -mediated cutaneous melanoma development and growth in vivo'. Together they form a unique fingerprint.

Cite this