TY - JOUR
T1 - Single pulses of cytoplasmic calcium associated with phagocytosis of individual zymosan particles by macrophages
AU - Meagher, Laura C.
AU - Moonga, Baljit S.
AU - Haslett, Christopher
AU - Huang, Christopher L.H.
AU - Zaidi, Mone
N1 - Funding Information:
AcknowIedmnents: The studies were supported in part by Arthritis and Rheumatism Council, UK (MZ), Leverhulme Trust, UK (MZ), British Lung Foundation, UK (CH) and Medical Research Council, UK (CH). We are grateful to Professor Iain MacIntyre for extending facilities. CLH-H thanks Mr. W. Smith for skilled assistance.
PY - 1991/5/31
Y1 - 1991/5/31
N2 - We have measured cytosolic free calcium levels ([Ca++]i) in individual macrophages during the phagocytosis of single zymosan particles. We report here that the contact of a macrophage with zymosan results in a rapid transient elevation of [Ca++]i. Each [Ca++]i pulse is symmetrical lasting for up to 30 seconds. In contrast, macrophage spreading is associated with repetitive [Ca++]i spiking occuring in salvos of up to four smaller spikes, each lasting for between 8 and 18 seconds. These qualitative and kinetic differences might suggest that the role of [Ca++]i in phagocytosis is distinct from its role in spreading.
AB - We have measured cytosolic free calcium levels ([Ca++]i) in individual macrophages during the phagocytosis of single zymosan particles. We report here that the contact of a macrophage with zymosan results in a rapid transient elevation of [Ca++]i. Each [Ca++]i pulse is symmetrical lasting for up to 30 seconds. In contrast, macrophage spreading is associated with repetitive [Ca++]i spiking occuring in salvos of up to four smaller spikes, each lasting for between 8 and 18 seconds. These qualitative and kinetic differences might suggest that the role of [Ca++]i in phagocytosis is distinct from its role in spreading.
UR - http://www.scopus.com/inward/record.url?scp=0025835510&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(91)92006-6
DO - 10.1016/0006-291X(91)92006-6
M3 - Article
C2 - 2043132
AN - SCOPUS:0025835510
SN - 0006-291X
VL - 177
SP - 460
EP - 465
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -