Single photon emission tomography measurement of benzodiazepine receptor number and affinity in primate brain: a constant infusion paradigm with [123I]iomazenil

Marc Laruelle; Anissa Abi-Dargham; Zachary Rattner; Mohammed S. Al-Tikriti; Yolanda Zea-Ponce; Sami S. Zoghbi; Dennis S. Charney; Julie Price; J. James Frost; Paul B. Hoffer; Ronald M. Baldwin; Robert B. Innis

doi:10.1016/0014-2999(93)90421-D

Single photon emission tomography measurement of benzodiazepine receptor number and affinity in primate brain: a constant infusion paradigm with [¹²³I]iomazenil

Marc Laruelle, Anissa Abi-Dargham, Zachary Rattner, Mohammed S. Al-Tikriti, Yolanda Zea-Ponce, Sami S. Zoghbi, Dennis S. Charney, Julie Price, J. James Frost, Paul B. Hoffer, Ronald M. Baldwin, Robert B. Innis

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Benzodiazepine receptor number and affinity were measured in vivo with single photon emission tomography (SPECT). Following an initial bolus injection, the radiotracer [¹²³I]iomazenil was infused at a constant rate for 5 to 8 h. This procedure induced a state of sustained equilibrium at the receptor level. Nondisplaceable activity was measured after injection of a receptor saturating dose of flumazenil. Experiments performed at high and low specific permitted estimation of an equilibrium binding affinity constant of 0.47 nM and a maximum binding capacity of 127 nM in occipital cortex.

Original language	English
Pages (from-to)	119-123
Number of pages	5
Journal	European Journal of Pharmacology
Volume	230
Issue number	1
DOIs	https://doi.org/10.1016/0014-2999(93)90421-D
State	Published - 5 Jan 1993
Externally published	Yes

Keywords

Benzodiazepine receptors
SPECT (single photon emission tomography)
[I]Iomazenil

Access to Document

10.1016/0014-2999(93)90421-D

Cite this

Laruelle, M., Abi-Dargham, A., Rattner, Z., Al-Tikriti, M. S., Zea-Ponce, Y., Zoghbi, S. S., Charney, D. S., Price, J., Frost, J. J., Hoffer, P. B., Baldwin, R. M., & Innis, R. B. (1993). Single photon emission tomography measurement of benzodiazepine receptor number and affinity in primate brain: a constant infusion paradigm with [¹²³I]iomazenil. European Journal of Pharmacology, 230(1), 119-123. https://doi.org/10.1016/0014-2999(93)90421-D

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title = "Single photon emission tomography measurement of benzodiazepine receptor number and affinity in primate brain: a constant infusion paradigm with [123I]iomazenil",

abstract = "Benzodiazepine receptor number and affinity were measured in vivo with single photon emission tomography (SPECT). Following an initial bolus injection, the radiotracer [123I]iomazenil was infused at a constant rate for 5 to 8 h. This procedure induced a state of sustained equilibrium at the receptor level. Nondisplaceable activity was measured after injection of a receptor saturating dose of flumazenil. Experiments performed at high and low specific permitted estimation of an equilibrium binding affinity constant of 0.47 nM and a maximum binding capacity of 127 nM in occipital cortex.",

keywords = "Benzodiazepine receptors, SPECT (single photon emission tomography), [I]Iomazenil",

author = "Marc Laruelle and Anissa Abi-Dargham and Zachary Rattner and Al-Tikriti, {Mohammed S.} and Yolanda Zea-Ponce and Zoghbi, {Sami S.} and Charney, {Dennis S.} and Julie Price and Frost, {J. James} and Hoffer, {Paul B.} and Baldwin, {Ronald M.} and Innis, {Robert B.}",

year = "1993",

month = jan,

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doi = "10.1016/0014-2999(93)90421-D",

language = "English",

volume = "230",

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journal = "European Journal of Pharmacology",

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Laruelle, M, Abi-Dargham, A, Rattner, Z, Al-Tikriti, MS, Zea-Ponce, Y, Zoghbi, SS, Charney, DS, Price, J, Frost, JJ, Hoffer, PB, Baldwin, RM & Innis, RB 1993, 'Single photon emission tomography measurement of benzodiazepine receptor number and affinity in primate brain: a constant infusion paradigm with [¹²³I]iomazenil', European Journal of Pharmacology, vol. 230, no. 1, pp. 119-123. https://doi.org/10.1016/0014-2999(93)90421-D

Single photon emission tomography measurement of benzodiazepine receptor number and affinity in primate brain: a constant infusion paradigm with [¹²³I]iomazenil. / Laruelle, Marc; Abi-Dargham, Anissa; Rattner, Zachary et al.
In: European Journal of Pharmacology, Vol. 230, No. 1, 05.01.1993, p. 119-123.

Research output: Contribution to journal › Article › peer-review

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AU - Abi-Dargham, Anissa

AU - Rattner, Zachary

AU - Al-Tikriti, Mohammed S.

AU - Zea-Ponce, Yolanda

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AU - Price, Julie

AU - Frost, J. James

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AU - Baldwin, Ronald M.

AU - Innis, Robert B.

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