@article{b6ed5d7ccfdb4fcdbdfadd6f56adab32,
title = "Single-cell RNA sequencing reveals maturation trajectory in human pluripotent stem cell-derived cardiomyocytes in engineered tissues",
abstract = "Cardiac in vitro models have become increasingly obtainable and affordable with the optimization of human pluripotent stem cell-derived cardiomyocyte (hPSC-CM) differentiation. However, these CMs are immature compared to their in vivo counterparts. Here we study the cellular phenotype of hPSC-CMs by comparing their single-cell gene expression and functional profiles in three engineered cardiac tissue configurations: human ventricular (hv) cardiac anisotropic sheet, cardiac tissue strip, and cardiac organoid chamber (hvCOC), with spontaneously aggregated 3D cardiac spheroids (CS) as control. The CM maturity was found to increase with increasing levels of complexity of the engineered tissues from CS to hvCOC. The contractile components are the first function to mature, followed by electrophysiology and oxidative metabolism. Notably, the 2D tissue constructs show a higher cellular organization whereas metabolic maturity preferentially increases in the 3D constructs. We conclude that the tissue engineering models resembling configurations of native tissues may be reliable for drug screening or disease modeling.",
keywords = "Cell biology, Stem cells research, Tissue Engineering",
author = "Shangli Cheng and David Breni{\`e}re-Letuffe and Virpi Ahola and Wong, {Andy O.T.} and Keung, {Hoi Yee} and Bimal Gurung and Zongli Zheng and Costa, {Kevin D.} and Lieu, {Deborah K.} and Wendy Keung and Li, {Ronald A.}",
note = "Funding Information: We thank the Genomics Core of the Centre for PanorOmic Sciences, the University of Hong Kong for scRNA-seq library preparation and sequencing. We thank Miller Leung and Renee Chou for their help in dissociating the tissues used for the scRNA-seq. Conceptualization, R.A.L. W.K. K.D.C. and D.K.L.; methodology, S.C. and V.A.; software; formal analysis; visualization, S.C.; validation, D.B-L and S.C; investigation, D.B-L, S.C. V.A. D.K.L, and W.K; resources, D.B-L, A.O.T.W, H.Y.K, B.G. and W.K; writing – original draft writing D.B-L, W.K. D.K.L, S.C. and V.A.; writing – review & editing, all authors; supervision, W.K. V.A. D.K.L, and Z.Z.; project administration, D.B-L, V.A. and W.K.; funding acquisition, R.A.L. K.D.C, and R.A.L. hold equities in Novoheart, whose value may potentially be affected by the publication of this article. S.C. D.B-L. V.A. A.O.T.W. H.Y.K, B.G. Z.Z. D.K.L, and W.K. have no competing interests. Publisher Copyright: {\textcopyright} 2023 The Author(s)",
year = "2023",
month = apr,
day = "21",
doi = "10.1016/j.isci.2023.106302",
language = "English",
volume = "26",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier Inc.",
number = "4",
}