Single and multiplexed fluorescent in situ hybridization using quantum dots nanocrystals in Xenopus

Andriani Ioannou; Iro Eleftheriou; Anna Charalambous; Paris A. Skourides

doi:10.1007/978-1-4939-2303-8_24

Single and multiplexed fluorescent in situ hybridization using quantum dots nanocrystals in Xenopus

Andriani Ioannou, Iro Eleftheriou, Anna Charalambous, Paris A. Skourides

Research output: Contribution to journal › Article › peer-review

Abstract

The photostability and narrow emission spectra of quantum dots (QDs) make them ideal candidates for whole-mount fluorescent in situ hybridization. Here we describe a method for non-amplified, single and multiplexed whole-mount fluorescent ISH in Xenopus laevis embryos using far red and near infrared (NIR) quantum dot nanocrystals. Our results demonstrate that QD detection of endogenous messenger RNA is effective even in highly autofluorescent organisms such as Xenopus and therefore holds promise for use in many other model organisms.

Original language	English
Pages (from-to)	451-462
Number of pages	12
Journal	Neuromethods
Volume	99
DOIs	https://doi.org/10.1007/978-1-4939-2303-8_24
State	Published - 2015

Keywords

Fluorescent whole-mount ISH
Multiplex
Nanocrystals
Quantum dots
Xenopus

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10.1007/978-1-4939-2303-8_24

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title = "Single and multiplexed fluorescent in situ hybridization using quantum dots nanocrystals in Xenopus",

abstract = "The photostability and narrow emission spectra of quantum dots (QDs) make them ideal candidates for whole-mount fluorescent in situ hybridization. Here we describe a method for non-amplified, single and multiplexed whole-mount fluorescent ISH in Xenopus laevis embryos using far red and near infrared (NIR) quantum dot nanocrystals. Our results demonstrate that QD detection of endogenous messenger RNA is effective even in highly autofluorescent organisms such as Xenopus and therefore holds promise for use in many other model organisms.",

keywords = "Fluorescent whole-mount ISH, Multiplex, Nanocrystals, Quantum dots, Xenopus",

author = "Andriani Ioannou and Iro Eleftheriou and Anna Charalambous and Skourides, {Paris A.}",

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doi = "10.1007/978-1-4939-2303-8_24",

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AU - Ioannou, Andriani

AU - Eleftheriou, Iro

AU - Charalambous, Anna

AU - Skourides, Paris A.

N1 - Publisher Copyright: © Springer Science+Business Media New York 2015.

PY - 2015

Y1 - 2015

N2 - The photostability and narrow emission spectra of quantum dots (QDs) make them ideal candidates for whole-mount fluorescent in situ hybridization. Here we describe a method for non-amplified, single and multiplexed whole-mount fluorescent ISH in Xenopus laevis embryos using far red and near infrared (NIR) quantum dot nanocrystals. Our results demonstrate that QD detection of endogenous messenger RNA is effective even in highly autofluorescent organisms such as Xenopus and therefore holds promise for use in many other model organisms.

AB - The photostability and narrow emission spectra of quantum dots (QDs) make them ideal candidates for whole-mount fluorescent in situ hybridization. Here we describe a method for non-amplified, single and multiplexed whole-mount fluorescent ISH in Xenopus laevis embryos using far red and near infrared (NIR) quantum dot nanocrystals. Our results demonstrate that QD detection of endogenous messenger RNA is effective even in highly autofluorescent organisms such as Xenopus and therefore holds promise for use in many other model organisms.

KW - Fluorescent whole-mount ISH

KW - Multiplex

KW - Nanocrystals

KW - Quantum dots

KW - Xenopus

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DO - 10.1007/978-1-4939-2303-8_24

M3 - Article

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SN - 0893-2336

VL - 99

SP - 451

EP - 462

JO - Neuromethods

JF - Neuromethods

ER -

Single and multiplexed fluorescent in situ hybridization using quantum dots nanocrystals in Xenopus

Abstract

Keywords

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