Simultaneous quantitative determination of olmesartan and hydrochlorothiazide in human plasma and urine by liquid chromatography coupled to tandem mass spectrometry

Dongyang Liu; Ji Jiang; Peng Wang; Sheng Feng; Pei Hu

doi:10.1016/j.jchromb.2010.01.009

Simultaneous quantitative determination of olmesartan and hydrochlorothiazide in human plasma and urine by liquid chromatography coupled to tandem mass spectrometry

Dongyang Liu
, Ji Jiang
, Peng Wang
, Sheng Feng
, Pei Hu

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

A specific, sensitive and rapid method based on high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) was developed for the simultaneous determination of olmesartan (OLM) and hydrochlorothiazide (HCTZ) in human plasma and urine. Solid-phase extraction (SPE) was used to isolate the analytes from biological matrices followed by injection of the extracts onto a C₁₈ column with isocratic elution. Detection was carried out on a triple quadrupole tandem mass spectrometer in multiple reaction monitoring (MRM) mode using negative electrospray ionization (ESI). The method was validated over the concentration range of 1.00-1000 ng/mL and 5.00-5000 ng/mL for OLM in human plasma and urine as well as 0.500-200 ng/mL and 25.0-25,000 ng/mL for HCTZ in human plasma and urine, respectively. Inter- and intra-run precision of OLM and HCTZ were less than 15% and the accuracy was within 85-115% for both plasma and urine. The average extraction recoveries were 96.6% and 92.7% for OLM, and 87.2% and 72.1% for HCTZ in human plasma and urine, respectively. The linearity, recovery, matrix effect and stability were validated for OLM/HCTZ in human plasma and urine.

Original language	English
Pages (from-to)	743-748
Number of pages	6
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	878
Issue number	9-10
DOIs	https://doi.org/10.1016/j.jchromb.2010.01.009
State	Published - 15 Mar 2010
Externally published	Yes

Keywords

Determination
HPLC-MS/MS
Human plasma and urine
Hydrochlorothiazide
Olmesartan
Simultaneous

Access to Document

10.1016/j.jchromb.2010.01.009

Cite this

Liu, D., Jiang, J., Wang, P., Feng, S., & Hu, P. (2010). Simultaneous quantitative determination of olmesartan and hydrochlorothiazide in human plasma and urine by liquid chromatography coupled to tandem mass spectrometry. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 878(9-10), 743-748. https://doi.org/10.1016/j.jchromb.2010.01.009

@article{f896b3e204324ef98139a95404596cf4,

title = "Simultaneous quantitative determination of olmesartan and hydrochlorothiazide in human plasma and urine by liquid chromatography coupled to tandem mass spectrometry",

abstract = "A specific, sensitive and rapid method based on high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) was developed for the simultaneous determination of olmesartan (OLM) and hydrochlorothiazide (HCTZ) in human plasma and urine. Solid-phase extraction (SPE) was used to isolate the analytes from biological matrices followed by injection of the extracts onto a C18 column with isocratic elution. Detection was carried out on a triple quadrupole tandem mass spectrometer in multiple reaction monitoring (MRM) mode using negative electrospray ionization (ESI). The method was validated over the concentration range of 1.00-1000 ng/mL and 5.00-5000 ng/mL for OLM in human plasma and urine as well as 0.500-200 ng/mL and 25.0-25,000 ng/mL for HCTZ in human plasma and urine, respectively. Inter- and intra-run precision of OLM and HCTZ were less than 15\% and the accuracy was within 85-115\% for both plasma and urine. The average extraction recoveries were 96.6\% and 92.7\% for OLM, and 87.2\% and 72.1\% for HCTZ in human plasma and urine, respectively. The linearity, recovery, matrix effect and stability were validated for OLM/HCTZ in human plasma and urine.",

keywords = "Determination, HPLC-MS/MS, Human plasma and urine, Hydrochlorothiazide, Olmesartan, Simultaneous",

author = "Dongyang Liu and Ji Jiang and Peng Wang and Sheng Feng and Pei Hu",

note = "Funding Information: This study was supported by the {\textquotedblleft}Eleventh Five-year{\textquotedblright} National Key Technology R\&D Program of China and Shanghai Sankyo Pharmaceutical Co., Ltd. (Shanghai, China). ",

year = "2010",

month = mar,

day = "15",

doi = "10.1016/j.jchromb.2010.01.009",

language = "English",

volume = "878",

pages = "743--748",

journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

issn = "1570-0232",

publisher = "Elsevier B.V.",

number = "9-10",

}

Liu, D, Jiang, J, Wang, P, Feng, S & Hu, P 2010, 'Simultaneous quantitative determination of olmesartan and hydrochlorothiazide in human plasma and urine by liquid chromatography coupled to tandem mass spectrometry', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 878, no. 9-10, pp. 743-748. https://doi.org/10.1016/j.jchromb.2010.01.009

Simultaneous quantitative determination of olmesartan and hydrochlorothiazide in human plasma and urine by liquid chromatography coupled to tandem mass spectrometry. / Liu, Dongyang; Jiang, Ji; Wang, Peng et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 878, No. 9-10, 15.03.2010, p. 743-748.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Simultaneous quantitative determination of olmesartan and hydrochlorothiazide in human plasma and urine by liquid chromatography coupled to tandem mass spectrometry

AU - Liu, Dongyang

AU - Jiang, Ji

AU - Wang, Peng

AU - Feng, Sheng

AU - Hu, Pei

N1 - Funding Information: This study was supported by the “Eleventh Five-year” National Key Technology R&D Program of China and Shanghai Sankyo Pharmaceutical Co., Ltd. (Shanghai, China).

PY - 2010/3/15

Y1 - 2010/3/15

N2 - A specific, sensitive and rapid method based on high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) was developed for the simultaneous determination of olmesartan (OLM) and hydrochlorothiazide (HCTZ) in human plasma and urine. Solid-phase extraction (SPE) was used to isolate the analytes from biological matrices followed by injection of the extracts onto a C18 column with isocratic elution. Detection was carried out on a triple quadrupole tandem mass spectrometer in multiple reaction monitoring (MRM) mode using negative electrospray ionization (ESI). The method was validated over the concentration range of 1.00-1000 ng/mL and 5.00-5000 ng/mL for OLM in human plasma and urine as well as 0.500-200 ng/mL and 25.0-25,000 ng/mL for HCTZ in human plasma and urine, respectively. Inter- and intra-run precision of OLM and HCTZ were less than 15% and the accuracy was within 85-115% for both plasma and urine. The average extraction recoveries were 96.6% and 92.7% for OLM, and 87.2% and 72.1% for HCTZ in human plasma and urine, respectively. The linearity, recovery, matrix effect and stability were validated for OLM/HCTZ in human plasma and urine.

AB - A specific, sensitive and rapid method based on high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) was developed for the simultaneous determination of olmesartan (OLM) and hydrochlorothiazide (HCTZ) in human plasma and urine. Solid-phase extraction (SPE) was used to isolate the analytes from biological matrices followed by injection of the extracts onto a C18 column with isocratic elution. Detection was carried out on a triple quadrupole tandem mass spectrometer in multiple reaction monitoring (MRM) mode using negative electrospray ionization (ESI). The method was validated over the concentration range of 1.00-1000 ng/mL and 5.00-5000 ng/mL for OLM in human plasma and urine as well as 0.500-200 ng/mL and 25.0-25,000 ng/mL for HCTZ in human plasma and urine, respectively. Inter- and intra-run precision of OLM and HCTZ were less than 15% and the accuracy was within 85-115% for both plasma and urine. The average extraction recoveries were 96.6% and 92.7% for OLM, and 87.2% and 72.1% for HCTZ in human plasma and urine, respectively. The linearity, recovery, matrix effect and stability were validated for OLM/HCTZ in human plasma and urine.

KW - Determination

KW - HPLC-MS/MS

KW - Human plasma and urine

KW - Hydrochlorothiazide

KW - Olmesartan

KW - Simultaneous

UR - https://www.scopus.com/pages/publications/77549088148

U2 - 10.1016/j.jchromb.2010.01.009

DO - 10.1016/j.jchromb.2010.01.009

M3 - Article

C2 - 20153270

AN - SCOPUS:77549088148

SN - 1570-0232

VL - 878

SP - 743

EP - 748

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 9-10

ER -

Simultaneous quantitative determination of olmesartan and hydrochlorothiazide in human plasma and urine by liquid chromatography coupled to tandem mass spectrometry

Abstract

Keywords

Access to Document

Fingerprint

Cite this