Simultaneous quantitation of fatty acids, sterols and bile acids in human stool by capillary gas-liquid chromatography

Ashok K. Batta; Gerald Salen; Priti Batta; G. Stephen Tint; David S. Alberts; David L. Earnest

doi:10.1016/S1570-0232(02)00289-1

Simultaneous quantitation of fatty acids, sterols and bile acids in human stool by capillary gas-liquid chromatography

Ashok K. Batta
, Gerald Salen
, Priti Batta
, G. Stephen Tint
, David S. Alberts
, David L. Earnest

Research output: Contribution to journal › Article › peer-review

69 Scopus citations

Abstract

A simple method for the simultaneous gas-liquid chromatographic quantitation of fatty acids, sterols and bile acids from human fecal samples is described. The various compounds are directly converted into the n-butyl ester-trimethylsilyl ether derivatives, without prior isolation from the stool. Under these conditions, fecal bile acid derivatives are well resolved from each other and from those of fecal fatty acids and sterols without overlaps. The method was found to be reproducible and recoveries were similar to those obtained after exhaustive solvent extraction of fecal sterols, fatty acids and bile acids. Optimum derivatization conditions that allowed maximum recovery of fecal components with minimal destruction and application of the method for simultaneous bile acid, fatty acid and sterol analysis in human stool are described.

Original language	English
Pages (from-to)	153-161
Number of pages	9
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	775
Issue number	2
DOIs	https://doi.org/10.1016/S1570-0232(02)00289-1
State	Published - 5 Aug 2002
Externally published	Yes

Keywords

Bile acids
Fatty acids
Sterols

Access to Document

10.1016/S1570-0232(02)00289-1

Cite this

@article{92679f2bf52d4b4e9c0413c2c07748ad,

title = "Simultaneous quantitation of fatty acids, sterols and bile acids in human stool by capillary gas-liquid chromatography",

abstract = "A simple method for the simultaneous gas-liquid chromatographic quantitation of fatty acids, sterols and bile acids from human fecal samples is described. The various compounds are directly converted into the n-butyl ester-trimethylsilyl ether derivatives, without prior isolation from the stool. Under these conditions, fecal bile acid derivatives are well resolved from each other and from those of fecal fatty acids and sterols without overlaps. The method was found to be reproducible and recoveries were similar to those obtained after exhaustive solvent extraction of fecal sterols, fatty acids and bile acids. Optimum derivatization conditions that allowed maximum recovery of fecal components with minimal destruction and application of the method for simultaneous bile acid, fatty acid and sterol analysis in human stool are described.",

keywords = "Bile acids, Fatty acids, Sterols",

author = "Batta, \{Ashok K.\} and Gerald Salen and Priti Batta and Tint, \{G. Stephen\} and Alberts, \{David S.\} and Earnest, \{David L.\}",

note = "Funding Information: This work was supported by grants from the Veterans Affairs Research Service, Washington, DC, USA and American Heart Association, New Jersey Affiliate, the National Institute of Health grant DK 568301A, and National Cancer Institute grant PO1-CA-41108. The contents of this publication are solely the responsibility of the authors and do not necessarily represent the official views of the National Cancer Institute.",

year = "2002",

month = aug,

day = "5",

doi = "10.1016/S1570-0232(02)00289-1",

language = "English",

volume = "775",

pages = "153--161",

journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

issn = "1570-0232",

publisher = "Elsevier B.V.",

number = "2",

}

Simultaneous quantitation of fatty acids, sterols and bile acids in human stool by capillary gas-liquid chromatography. / Batta, Ashok K.; Salen, Gerald; Batta, Priti et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 775, No. 2, 05.08.2002, p. 153-161.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Simultaneous quantitation of fatty acids, sterols and bile acids in human stool by capillary gas-liquid chromatography

AU - Batta, Ashok K.

AU - Salen, Gerald

AU - Batta, Priti

AU - Tint, G. Stephen

AU - Alberts, David S.

AU - Earnest, David L.

N1 - Funding Information: This work was supported by grants from the Veterans Affairs Research Service, Washington, DC, USA and American Heart Association, New Jersey Affiliate, the National Institute of Health grant DK 568301A, and National Cancer Institute grant PO1-CA-41108. The contents of this publication are solely the responsibility of the authors and do not necessarily represent the official views of the National Cancer Institute.

PY - 2002/8/5

Y1 - 2002/8/5

N2 - A simple method for the simultaneous gas-liquid chromatographic quantitation of fatty acids, sterols and bile acids from human fecal samples is described. The various compounds are directly converted into the n-butyl ester-trimethylsilyl ether derivatives, without prior isolation from the stool. Under these conditions, fecal bile acid derivatives are well resolved from each other and from those of fecal fatty acids and sterols without overlaps. The method was found to be reproducible and recoveries were similar to those obtained after exhaustive solvent extraction of fecal sterols, fatty acids and bile acids. Optimum derivatization conditions that allowed maximum recovery of fecal components with minimal destruction and application of the method for simultaneous bile acid, fatty acid and sterol analysis in human stool are described.

AB - A simple method for the simultaneous gas-liquid chromatographic quantitation of fatty acids, sterols and bile acids from human fecal samples is described. The various compounds are directly converted into the n-butyl ester-trimethylsilyl ether derivatives, without prior isolation from the stool. Under these conditions, fecal bile acid derivatives are well resolved from each other and from those of fecal fatty acids and sterols without overlaps. The method was found to be reproducible and recoveries were similar to those obtained after exhaustive solvent extraction of fecal sterols, fatty acids and bile acids. Optimum derivatization conditions that allowed maximum recovery of fecal components with minimal destruction and application of the method for simultaneous bile acid, fatty acid and sterol analysis in human stool are described.

KW - Bile acids

KW - Fatty acids

KW - Sterols

UR - https://www.scopus.com/pages/publications/0037025852

U2 - 10.1016/S1570-0232(02)00289-1

DO - 10.1016/S1570-0232(02)00289-1

M3 - Article

C2 - 12113981

AN - SCOPUS:0037025852

SN - 1570-0232

VL - 775

SP - 153

EP - 161

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 2

ER -

Simultaneous quantitation of fatty acids, sterols and bile acids in human stool by capillary gas-liquid chromatography

Abstract

Keywords

Access to Document

Fingerprint

Cite this