Simultaneous determination of acetaminophen, dextromethorphen hydrobromide and pseudoephedrine hydrochloride in a new drug formulation for cold treatment by HPLC

M. L. Qi, P. Wang, L. Zhou, J. L. Gu, R. N. Fu

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7 Scopus citations

Abstract

A rapid and accurate HPLC method is described for the simultaneous determination of acetaminophen, dextromethorphen hydrobromide and pseudoephedrine hydrochloride in a new cold formulation. Chromatographic separation of the three pharmaceuticals was performed on a Hypersil CN column (150 × 5.0 mm, 5 μm) with a mobile phase mixture of an ion-pairing solution, methanol and acetonitrile (25:57:18, v/v), at a flow rate of 1.0 mL min-1, with detection at 220 nm. Separation was complete in less than 10 min. The method was validated for linearity, accuracy, precision, limit of quantitation and robustness. Linearity, accuracy, and precision were found to be acceptable over the ranges of 2.06 ∼ 20.6 μg·mL-1 for acetaminophen, 0.202 ∼ 2.02 mg·mL-1 for pseudoephedrine hydrochloride and 0.042 ∼ 1.06 mg·mL-1 for dextromethorphen hydrobromide.

Original languageEnglish
Pages (from-to)139-142
Number of pages4
JournalChromatographia
Volume57
Issue number3-4
DOIs
StatePublished - Feb 2003
Externally publishedYes

Keywords

  • Acetaminophen
  • Cold formulation
  • Column liquid chromatography
  • Dextromethorphen hydrobromide
  • Pseudoephedrine hydrochbride

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