Simultaneous determination of 3,4-dihydroxyphenylacetic acid and homovanillic acid in milligram amounts of rat striatal tissue by gas-liquid chromatography

Eric Watson; Barbara Travis; Sherwin Wilk

doi:10.1016/0024-3205(74)90033-2

Simultaneous determination of 3,4-dihydroxyphenylacetic acid and homovanillic acid in milligram amounts of rat striatal tissue by gas-liquid chromatography

Eric Watson
, Barbara Travis
, Sherwin Wilk

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

Simultaneous determination of the major metabolites of dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in rat striatum has been achieved by gas-liquid chromatography. Striatal tissue from one rat was homogenized in IN HCl and one-tenth of the sample extracted with ethyl ether. After evaporation of the ether, the residue was reacted with a combination of 1-chloro-1,1,3,3,3-pentafluor-2-propanol and pentafluoropropionic anhydride followed by reaction with pentafluoropropionic anhydride. The derivatives were chromatographed on a 3% JXR column and quantitated using electron capture detection. The propionic homologs of DOPAC and HVA served as internal standards. The steady state levels of DOPAC and HVA were found to be 0.90 μg/gm±0.21 S.D. (N=12) and 0.66 μg/gm±0.16 S.D. (N=12) respectively.

Original language	English
Pages (from-to)	2167-2178
Number of pages	12
Journal	Life Sciences
Volume	15
Issue number	12
DOIs	https://doi.org/10.1016/0024-3205(74)90033-2
State	Published - 15 Dec 1974
Externally published	Yes

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title = "Simultaneous determination of 3,4-dihydroxyphenylacetic acid and homovanillic acid in milligram amounts of rat striatal tissue by gas-liquid chromatography",

abstract = "Simultaneous determination of the major metabolites of dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in rat striatum has been achieved by gas-liquid chromatography. Striatal tissue from one rat was homogenized in IN HCl and one-tenth of the sample extracted with ethyl ether. After evaporation of the ether, the residue was reacted with a combination of 1-chloro-1,1,3,3,3-pentafluor-2-propanol and pentafluoropropionic anhydride followed by reaction with pentafluoropropionic anhydride. The derivatives were chromatographed on a 3\% JXR column and quantitated using electron capture detection. The propionic homologs of DOPAC and HVA served as internal standards. The steady state levels of DOPAC and HVA were found to be 0.90 μg/gm±0.21 S.D. (N=12) and 0.66 μg/gm±0.16 S.D. (N=12) respectively.",

author = "Eric Watson and Barbara Travis and Sherwin Wilk",

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doi = "10.1016/0024-3205(74)90033-2",

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T1 - Simultaneous determination of 3,4-dihydroxyphenylacetic acid and homovanillic acid in milligram amounts of rat striatal tissue by gas-liquid chromatography

AU - Watson, Eric

AU - Travis, Barbara

AU - Wilk, Sherwin

N1 - Funding Information: minergic theory of schizophrenia is supported by the ability of

PY - 1974/12/15

Y1 - 1974/12/15

N2 - Simultaneous determination of the major metabolites of dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in rat striatum has been achieved by gas-liquid chromatography. Striatal tissue from one rat was homogenized in IN HCl and one-tenth of the sample extracted with ethyl ether. After evaporation of the ether, the residue was reacted with a combination of 1-chloro-1,1,3,3,3-pentafluor-2-propanol and pentafluoropropionic anhydride followed by reaction with pentafluoropropionic anhydride. The derivatives were chromatographed on a 3% JXR column and quantitated using electron capture detection. The propionic homologs of DOPAC and HVA served as internal standards. The steady state levels of DOPAC and HVA were found to be 0.90 μg/gm±0.21 S.D. (N=12) and 0.66 μg/gm±0.16 S.D. (N=12) respectively.

AB - Simultaneous determination of the major metabolites of dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) in rat striatum has been achieved by gas-liquid chromatography. Striatal tissue from one rat was homogenized in IN HCl and one-tenth of the sample extracted with ethyl ether. After evaporation of the ether, the residue was reacted with a combination of 1-chloro-1,1,3,3,3-pentafluor-2-propanol and pentafluoropropionic anhydride followed by reaction with pentafluoropropionic anhydride. The derivatives were chromatographed on a 3% JXR column and quantitated using electron capture detection. The propionic homologs of DOPAC and HVA served as internal standards. The steady state levels of DOPAC and HVA were found to be 0.90 μg/gm±0.21 S.D. (N=12) and 0.66 μg/gm±0.16 S.D. (N=12) respectively.

UR - https://www.scopus.com/pages/publications/0016394355

U2 - 10.1016/0024-3205(74)90033-2

DO - 10.1016/0024-3205(74)90033-2

M3 - Article

C2 - 4621013

AN - SCOPUS:0016394355

SN - 0024-3205

VL - 15

SP - 2167

EP - 2178

JO - Life Sciences

JF - Life Sciences

IS - 12

ER -

Simultaneous determination of 3,4-dihydroxyphenylacetic acid and homovanillic acid in milligram amounts of rat striatal tissue by gas-liquid chromatography

Abstract

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