TY - JOUR
T1 - Simultaneous detection of salivary Δ9-tetrahydrocannabinol and alcohol using a Wearable Electrochemical Ring Sensor
AU - Mishra, Rupesh K.
AU - Sempionatto, Juliane R.
AU - Li, Zhanhong
AU - Brown, Christopher
AU - Galdino, Nathalia M.
AU - Shah, Rushabh
AU - Liu, Shuyang
AU - Hubble, Lee J.
AU - Bagot, Kara
AU - Tapert, Susan
AU - Wang, Joseph
N1 - Funding Information:
Financial support from The Defence Threat Reduction Agency Joint Science and Technology Office for Chemical and Biological Defence ( HDTRA 1-16-1-0013 ) and the UCSD Centre of Wearable Sensors is acknowledged. J.R.S. acknowledges support from the National Council for Scientific and Technological Development ( CNPq-216981/2014-0 ). R.K.M. acknowledges a fellowship from Department of Biotechnology, New Delhi, India. N.M.G. acknowledges CAPES scholarship/PDSE/number 88881.188746/2018–01 . L.J.H. acknowledges support from the Research Plus Julius Career Award by CSIRO and Z.L. acknowledges support from the 2018 Shanghai Young and Middle-aged Teachers' Foreign Visiting Program.
Funding Information:
Financial support from The Defence Threat Reduction Agency Joint Science and Technology Office for Chemical and Biological Defence (HDTRA 1-16-1-0013) and the UCSD Centre of Wearable Sensors is acknowledged. J.R.S. acknowledges support from the National Council for Scientific and Technological Development (CNPq-216981/2014-0). R.K.M. acknowledges a fellowship from Department of Biotechnology, New Delhi, India. N.M.G. acknowledges CAPES scholarship/PDSE/number 88881.188746/2018?01. L.J.H. acknowledges support from the Research Plus Julius Career Award by CSIRO and Z.L. acknowledges support from the 2018 Shanghai Young and Middle-aged Teachers' Foreign Visiting Program.
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/5/1
Y1 - 2020/5/1
N2 - Driving under the influence of cannabis and alcohol represents a major safety concern due to the synergistic or additive effect of these substances of abuse. Hence, rapid road-site testing of these substances is highly desired to reduce risks of fatal accidents. Here we describe a wearable electrochemical sensing device for the simultaneous direct, decentralized, detection of salivary THC and alcohol. The new ring-based sensing platform contains a voltammetric THC sensor and an amperometric alcohol biosensor on the ring cap, along with the wireless electronics embedded within the ring case. Rapid replacement of the disposable sensing-electrode ring cap following each saliva assay is accomplished by aligning spring-loaded pins, mounted on the electronic board (PCB), with the current collectors of the sensing electrodes. The printed dual-analyte sensor ring cover is based on a MWCNT/carbon electrode for the THC detection along with a Prussian-blue transducer, coated with alcohol oxidase/chitosan reagent layer, for the biosensing of alcohol. THC and alcohol can thus be detected simultaneously in the same diluted saliva sample within 3 min, with no cross talk and no interferences from the saliva matrix. The new wearable ring sensor platform should enable law enforcement personnel to screen drivers in a single traffic stop and offers considerable promise for addressing growing concerns of drug-impaired driving.
AB - Driving under the influence of cannabis and alcohol represents a major safety concern due to the synergistic or additive effect of these substances of abuse. Hence, rapid road-site testing of these substances is highly desired to reduce risks of fatal accidents. Here we describe a wearable electrochemical sensing device for the simultaneous direct, decentralized, detection of salivary THC and alcohol. The new ring-based sensing platform contains a voltammetric THC sensor and an amperometric alcohol biosensor on the ring cap, along with the wireless electronics embedded within the ring case. Rapid replacement of the disposable sensing-electrode ring cap following each saliva assay is accomplished by aligning spring-loaded pins, mounted on the electronic board (PCB), with the current collectors of the sensing electrodes. The printed dual-analyte sensor ring cover is based on a MWCNT/carbon electrode for the THC detection along with a Prussian-blue transducer, coated with alcohol oxidase/chitosan reagent layer, for the biosensing of alcohol. THC and alcohol can thus be detected simultaneously in the same diluted saliva sample within 3 min, with no cross talk and no interferences from the saliva matrix. The new wearable ring sensor platform should enable law enforcement personnel to screen drivers in a single traffic stop and offers considerable promise for addressing growing concerns of drug-impaired driving.
KW - Alcohol sensor
KW - Electrochemical sensor
KW - Marijuana sensor
KW - Ring sensor
KW - Saliva sensor
KW - Wearable THC sensor
UR - http://www.scopus.com/inward/record.url?scp=85078506413&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2020.120757
DO - 10.1016/j.talanta.2020.120757
M3 - Article
C2 - 32070607
AN - SCOPUS:85078506413
VL - 211
JO - Talanta
JF - Talanta
SN - 0039-9140
M1 - 120757
ER -