Simulated microgravity significantly altered metabolism in epidermal stem cells

Bin Bin Li, Zheng Yang Chen, Nan Jiang, Song Guo, Jia Qi Yang, Shao Bin Chai, Hong Feng Yan, Pei Ming Sun, Gang Hu, Tao Zhang, Bing Xin Xu, Hong Wei Sun, Jin Lian Zhou, He Ming Yang, Yan Cui

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Simulated microgravity can significantly affect various cell types and multiple systems of the human body, such as cardiovascular system, skeletal muscle system, and immune system, and is known to cause anemia and loss of electrolyte and fluids. Epidermal stem cells (EpSCs) were cultured in a rotary cell culture system (RCCS) bioreactor to simulate microgravity. The metabolites of EpSCs were identified by liquid chromatography-mass spectrometry (LC-MS). Compared with normal gravity (NG) group, a total of 57 different metabolites of EpSCs were identified (P < 0.05, VIP > 1), including lipids and lipid-like molecules (51 molecules), amino acids (5 molecules), nucleosides, nucleotides, and analogues (1 molecule). According to the partial least squares discriminant analysis (PLS-DA) score plot, a VIP > 1 and P < 0.05 were obtained for the 57 different metabolites, of which 23 molecules were significantly downregulated and 34 were significantly upregulated in simulated microgravity (SMG) group. These results showed that SMG has a significant impact on different pathways, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated that multiple pathways were involved, mainly the amino acid metabolism pathway, lipid metabolism pathway, membrane transport pathway, and cell growth and death pathways. Thus, the metabolic profile of EpSCs was changed under SMG. Exploring the metabolic profile of EpSCs would be helpful to further understand the growth characteristics of EpSCs under SMG, which will provide a new approach to explore the metabolomics mechanism of stress injury and repair trauma under SMG.

Original languageEnglish
Pages (from-to)200-212
Number of pages13
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume56
Issue number3
DOIs
StatePublished - 1 Mar 2020
Externally publishedYes

Keywords

  • Epidermal stem cells
  • Metabolism
  • Metabolism pathway
  • RCCS
  • Simulated microgravity

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