Simple differential digital confocal aperture to improve axial response of line-scanning confocal microendoscopes

Yubo Tang, Alex Kortum, Imran Vohra, Jennifer Carns, Sharmila Anandasabapathy, Rebecca Richards-Kortum

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Line-scanning confocal microendoscopy offers video-rate cellular imaging of scattering tissue with relatively simple hardware, but its axial response is inferior to that of point-scanning systems. Based on Fourier optics theory, we designed differential confocal apertures with a simple subtraction technique to improve the line-scanning sectioning performance. Taking advantage of digital slit apertures on a digital light projector and a CMOS rolling shutter, we demonstrate real-time optical sectioning performance comparable to point scanning in a dual-camera microendoscope (<$6, 000). We validate the background rejection capability when imaging porcine columnar epithelium stained with fluorescent contrast agents with different uptake mechanisms and staining properties.

Original languageEnglish
Pages (from-to)4519-4522
Number of pages4
JournalOptics Letters
Volume44
Issue number18
DOIs
StatePublished - 15 Sep 2019
Externally publishedYes

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