TY - JOUR
T1 - Similarity of an estrogen-induced protein and a luteinizing hormone releasing hormone-induced protein
AU - Mobbs, C.
AU - Fink, G.
AU - Johnson, M.
AU - Welch, W.
AU - Pfaff, D.
N1 - Funding Information:
This work was supported by a grant from the National Science Foundation (BNS-8716032) to C.V.M. with additional support to W.J.W. (NIH grant GM33551). Radioimmunoassay materials were generously provided by the National Hormone and Pituitary Program of the NIDDKK (Baltimore, MD, U.S.A.) and the Scottish Antibody Production Unit, Carluke, Scotland, U.K. We appreciate the superb technical assistance of Marianne Marquardt.
PY - 1989/4
Y1 - 1989/4
N2 - Estradiol induces a 70 kDa protein ('EI70') which is synthesized in vivo in the female rat ventromedial hypothalamus (VMH) and transported to the midbrain central gray, suggesting a role for EI70 in the female mating behavior, lordosis. Luteinizing hormone releasing hormone (LHRH), in addition to stimulating gonadotropin release, potentiates pituitary responsiveness to subsequent exposure to LHRH (the 'priming' effect), facilitates lordosis and induces the synthesis of a 70 kDa protein ('LHRH70') in pituitary in vitro. We now report that EI70 precisely co-migrates on two-dimensional (2-D) gels with the pituitary protein induced by LHRH both in vitro and in vivo. Furthermore, both proteins migrate on 2-D gels in the vicinity of a protein recognized after immunoblotting by antibodies to the heat-shock-70 kDa protein family. The induction of a common protein by estrogen or LHRH could represent a common mechanism by which these hormones facilitate secretion, and by which these hormones interact.
AB - Estradiol induces a 70 kDa protein ('EI70') which is synthesized in vivo in the female rat ventromedial hypothalamus (VMH) and transported to the midbrain central gray, suggesting a role for EI70 in the female mating behavior, lordosis. Luteinizing hormone releasing hormone (LHRH), in addition to stimulating gonadotropin release, potentiates pituitary responsiveness to subsequent exposure to LHRH (the 'priming' effect), facilitates lordosis and induces the synthesis of a 70 kDa protein ('LHRH70') in pituitary in vitro. We now report that EI70 precisely co-migrates on two-dimensional (2-D) gels with the pituitary protein induced by LHRH both in vitro and in vivo. Furthermore, both proteins migrate on 2-D gels in the vicinity of a protein recognized after immunoblotting by antibodies to the heat-shock-70 kDa protein family. The induction of a common protein by estrogen or LHRH could represent a common mechanism by which these hormones facilitate secretion, and by which these hormones interact.
KW - Estrogen
KW - Heat-shock-70 kDa protein
KW - Hypothalamus
KW - Luteinizing hormone-releasing hormone
KW - Pituitary
UR - https://www.scopus.com/pages/publications/0024591580
U2 - 10.1016/0303-7207(89)90017-8
DO - 10.1016/0303-7207(89)90017-8
M3 - Article
C2 - 2663572
AN - SCOPUS:0024591580
SN - 0303-7207
VL - 62
SP - 297
EP - 306
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
IS - 2
ER -