Silencing of PMT expression caused a surge of anatabine accumulation in tobacco

Peng Wang, Jia Zeng, Zhifeng Liang, Zhiqi Miao, Xiaofen Sun, Kexuan Tang

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Drastic increase of anatabine levels was observed in tobacco plants with markedly reduced nicotine concentrations through RNA silencing approaches. By down-regulation of PMT through three kinds of RNA silencing approaches, the nicotine levels decreased accordingly. In lines with slight and moderate reduction of nicotine levels, no anticipated negative linear correlation was found between anatabine and nicotine content. In lines with nicotine levels lower than 2.7 mg/g, drastic elevation of anatabine levels was found. Transcriptional levels of QPRT were unaffected in tobacco lines with surged anatabine levels. This report of an intriguing mutual relationship of nicotine and anatabine sheds new light on mechanisms between metabolic regulations in plants, and reconfirms complexity of metabolic networks.

Original languageEnglish
Pages (from-to)2285-2289
Number of pages5
JournalMolecular Biology Reports
Volume36
Issue number8
DOIs
StatePublished - Nov 2009
Externally publishedYes

Keywords

  • Anatabine
  • Metabolic regulation
  • Nicotine
  • Putrescine N-methyl Transferase (PMT)
  • Quinolinate Phosphoribosyl Transferase (QPRT)
  • Secondary metabolism
  • Tobacco

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