Signalling effects induced by acid ceramidase in human epithelial or leukemic cell lines

Käthe Baduva, Lara Büchter, Katja Kreyenkamp, Linnea Westphal, Barbara Wilker, Marcus Kohnen, Edward H. Schuchman, Michael J. Edwards, Katrin Anne Becker, Erich Gulbins, Alexander Carpinteiro

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Background/Aims: Recent studies indicated that an inhalation treatment of cystic fibrosis mice with acid ceramidase prevents and eliminates infections with Pseudomonas aeruginosa and Stapyhlococcus aureus. Inhalation of acid ceramidase facilitated the elimination of P. aeruginosa in acutely- or chronically-infected mice with cystic fibrosis. Thus, inhalation of acid ceramidase might be a preventive and/or curative treatment for patients with cystic fibrosis suffering from pneumonia. Methods: We treated cultured epithelial cells or leukemic T-lymphocytes (Jurkat cells) with purified acid ceramidase and determined intracellular signalling events, proliferation and cell survival. Specifically, we measured the activity of AKT, p38-kinase and p70S6-kinase using activation-specific phospho-antibodies in western blot studies. Trypan Blue staining served to analyze proliferation and cell survival. Results: Our studies indicate that treatment of Chang epithelial cells or Jurkat T lymphocytes with purified acid ceramidase results in a dose dependent activation of AKT, p38-kinase and p70S6-kinase, while tyrosine phosphorylation of intracellular proteins remains largely unchanged. Acid ceramidase treatment did not change expression of tight junction proteins such as ZO-1, ZO-2 and occludin. Cellular viability and proliferation were not affected by acid ceramidase treatment. Conclusion: Our data suggest that treatment of epithelial cells and lymphocytes with acid ceramidase results in activation of distinct pathways, in particular AKT- and p38K-dependent pathways, while no global activation or cell death was observed.

Original languageEnglish
Pages (from-to)1092-1102
Number of pages11
JournalCellular Physiology and Biochemistry
Issue number5
StatePublished - 2019


  • Acid ceramidase
  • Cystic fibrosis
  • Epithelial cells
  • Lymphocytes
  • Signalling


Dive into the research topics of 'Signalling effects induced by acid ceramidase in human epithelial or leukemic cell lines'. Together they form a unique fingerprint.

Cite this