Signaling protein SWAP-70 is required for efficient B cell homing to lymphoid organs

Glen Pearce; Veronique Angeli; Gwendalyn J. Randolph; Tobias Junt; Ulrich von Andrian; Hans Joachim Schnittler; Rolf Jessberger

doi:10.1038/ni1365

Signaling protein SWAP-70 is required for efficient B cell homing to lymphoid organs

Glen Pearce, Veronique Angeli, Gwendalyn J. Randolph, Tobias Junt, Ulrich von Andrian, Hans Joachim Schnittler, Rolf Jessberger

Icahn School of Medicine at Mount Sinai

Research output: Contribution to journal › Article › peer-review

67 Scopus citations

Abstract

The migration of B cells into secondary lymphoid organs is required for the generation of an effective immune response. Here we analyzed the involvement of SWAP-70, a Rac-interacting protein involved in actin rearrangement, in B cell entry into lymph nodes. We noted reduced migration of Swap70^-/- B cells into lymph nodes in vivo. Swap70^-/- B cells rolled and adhered, yet accumulated in lymph node high endothelial venules. This defect was not due to impaired integrin expression or chemotaxis. Instead, Swap70^-/- B cells aberrantly regulated integrin-mediated adhesion. During attachment, Swap70^-/- B cells showed defective polarization and did not form uropods or stabilize lamellipodia at a defined region. Thus, SWAP-70 selectively regulates processes essential for B cell entry into lymph nodes.

Original language	English
Pages (from-to)	827-834
Number of pages	8
Journal	Nature Immunology
Volume	7
Issue number	8
DOIs	https://doi.org/10.1038/ni1365
State	Published - Aug 2006

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title = "Signaling protein SWAP-70 is required for efficient B cell homing to lymphoid organs",

abstract = "The migration of B cells into secondary lymphoid organs is required for the generation of an effective immune response. Here we analyzed the involvement of SWAP-70, a Rac-interacting protein involved in actin rearrangement, in B cell entry into lymph nodes. We noted reduced migration of Swap70-/- B cells into lymph nodes in vivo. Swap70-/- B cells rolled and adhered, yet accumulated in lymph node high endothelial venules. This defect was not due to impaired integrin expression or chemotaxis. Instead, Swap70-/- B cells aberrantly regulated integrin-mediated adhesion. During attachment, Swap70-/- B cells showed defective polarization and did not form uropods or stabilize lamellipodia at a defined region. Thus, SWAP-70 selectively regulates processes essential for B cell entry into lymph nodes.",

author = "Glen Pearce and Veronique Angeli and Randolph, {Gwendalyn J.} and Tobias Junt and {von Andrian}, Ulrich and Schnittler, {Hans Joachim} and Rolf Jessberger",

note = "Funding Information: We thank L. Quemeneur for discussions, and A. Berg and M. Chopin for help. Supported by the National Institutes of Health (AI49470 to R.J.) and the Deutsche Forschungsgemeinschaft (SFB605 to R.J.).",

year = "2006",

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doi = "10.1038/ni1365",

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T1 - Signaling protein SWAP-70 is required for efficient B cell homing to lymphoid organs

AU - Pearce, Glen

AU - Angeli, Veronique

AU - Randolph, Gwendalyn J.

AU - Junt, Tobias

AU - von Andrian, Ulrich

AU - Schnittler, Hans Joachim

AU - Jessberger, Rolf

N1 - Funding Information: We thank L. Quemeneur for discussions, and A. Berg and M. Chopin for help. Supported by the National Institutes of Health (AI49470 to R.J.) and the Deutsche Forschungsgemeinschaft (SFB605 to R.J.).

PY - 2006/8

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N2 - The migration of B cells into secondary lymphoid organs is required for the generation of an effective immune response. Here we analyzed the involvement of SWAP-70, a Rac-interacting protein involved in actin rearrangement, in B cell entry into lymph nodes. We noted reduced migration of Swap70-/- B cells into lymph nodes in vivo. Swap70-/- B cells rolled and adhered, yet accumulated in lymph node high endothelial venules. This defect was not due to impaired integrin expression or chemotaxis. Instead, Swap70-/- B cells aberrantly regulated integrin-mediated adhesion. During attachment, Swap70-/- B cells showed defective polarization and did not form uropods or stabilize lamellipodia at a defined region. Thus, SWAP-70 selectively regulates processes essential for B cell entry into lymph nodes.

AB - The migration of B cells into secondary lymphoid organs is required for the generation of an effective immune response. Here we analyzed the involvement of SWAP-70, a Rac-interacting protein involved in actin rearrangement, in B cell entry into lymph nodes. We noted reduced migration of Swap70-/- B cells into lymph nodes in vivo. Swap70-/- B cells rolled and adhered, yet accumulated in lymph node high endothelial venules. This defect was not due to impaired integrin expression or chemotaxis. Instead, Swap70-/- B cells aberrantly regulated integrin-mediated adhesion. During attachment, Swap70-/- B cells showed defective polarization and did not form uropods or stabilize lamellipodia at a defined region. Thus, SWAP-70 selectively regulates processes essential for B cell entry into lymph nodes.

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ER -

Signaling protein SWAP-70 is required for efficient B cell homing to lymphoid organs

Abstract

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