Short-term clearance of cell-free nucleic acids after first-trimester termination of pregnancy

Young Ju Jeong; Lynn Borgatta; Nathalie Kapp; Inga Peter; Diana W. Bianchi; Kirby L. Johnson

doi:10.1016/j.fertnstert.2006.11.143

Short-term clearance of cell-free nucleic acids after first-trimester termination of pregnancy

Young Ju Jeong, Lynn Borgatta, Nathalie Kapp, Inga Peter, Diana W. Bianchi, Kirby L. Johnson

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

To determine the kinetics of the short-term clearance of cell-free nucleic acids in maternal plasma, we conducted a prospective cohort study of 36 women who underwent first-trimester elective surgical termination. By using real-time polymerase chain reaction (PCR) amplification and measurement of the sex-determining region of the Y chromosome (SRY) gene (as a marker of fetal DNA), β-hCG (a placental messenger RNA transcript), and glyceraldehyde phosphate dehydrogenase (GAPDH; as a marker of both total DNA and mitochondrial RNA), we found that cell-free nucleic acids in maternal plasma are not cleared within 90 minutes after the procedure, in contrast to the case of term delivery, in which levels decrease rapidly after birth.

Original language	English
Pages (from-to)	730-733
Number of pages	4
Journal	Fertility and Sterility
Volume	88
Issue number	3
DOIs	https://doi.org/10.1016/j.fertnstert.2006.11.143
State	Published - Sep 2007
Externally published	Yes

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title = "Short-term clearance of cell-free nucleic acids after first-trimester termination of pregnancy",

abstract = "To determine the kinetics of the short-term clearance of cell-free nucleic acids in maternal plasma, we conducted a prospective cohort study of 36 women who underwent first-trimester elective surgical termination. By using real-time polymerase chain reaction (PCR) amplification and measurement of the sex-determining region of the Y chromosome (SRY) gene (as a marker of fetal DNA), β-hCG (a placental messenger RNA transcript), and glyceraldehyde phosphate dehydrogenase (GAPDH; as a marker of both total DNA and mitochondrial RNA), we found that cell-free nucleic acids in maternal plasma are not cleared within 90 minutes after the procedure, in contrast to the case of term delivery, in which levels decrease rapidly after birth.",

author = "Jeong, {Young Ju} and Lynn Borgatta and Nathalie Kapp and Inga Peter and Bianchi, {Diana W.} and Johnson, {Kirby L.}",

note = "Funding Information: Supported by a National Institutes of Health (Bethesda, Maryland) grant (D.W.B.; R01 HD42053), as well as by a Korea Research Foundation (Seoul, South Korea) grant (Y-JJ; 2004-214-100235). ",

year = "2007",

month = sep,

doi = "10.1016/j.fertnstert.2006.11.143",

language = "English",

volume = "88",

pages = "730--733",

journal = "Fertility and Sterility",

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TY - JOUR

T1 - Short-term clearance of cell-free nucleic acids after first-trimester termination of pregnancy

AU - Jeong, Young Ju

AU - Borgatta, Lynn

AU - Kapp, Nathalie

AU - Peter, Inga

AU - Bianchi, Diana W.

AU - Johnson, Kirby L.

N1 - Funding Information: Supported by a National Institutes of Health (Bethesda, Maryland) grant (D.W.B.; R01 HD42053), as well as by a Korea Research Foundation (Seoul, South Korea) grant (Y-JJ; 2004-214-100235).

PY - 2007/9

Y1 - 2007/9

N2 - To determine the kinetics of the short-term clearance of cell-free nucleic acids in maternal plasma, we conducted a prospective cohort study of 36 women who underwent first-trimester elective surgical termination. By using real-time polymerase chain reaction (PCR) amplification and measurement of the sex-determining region of the Y chromosome (SRY) gene (as a marker of fetal DNA), β-hCG (a placental messenger RNA transcript), and glyceraldehyde phosphate dehydrogenase (GAPDH; as a marker of both total DNA and mitochondrial RNA), we found that cell-free nucleic acids in maternal plasma are not cleared within 90 minutes after the procedure, in contrast to the case of term delivery, in which levels decrease rapidly after birth.

AB - To determine the kinetics of the short-term clearance of cell-free nucleic acids in maternal plasma, we conducted a prospective cohort study of 36 women who underwent first-trimester elective surgical termination. By using real-time polymerase chain reaction (PCR) amplification and measurement of the sex-determining region of the Y chromosome (SRY) gene (as a marker of fetal DNA), β-hCG (a placental messenger RNA transcript), and glyceraldehyde phosphate dehydrogenase (GAPDH; as a marker of both total DNA and mitochondrial RNA), we found that cell-free nucleic acids in maternal plasma are not cleared within 90 minutes after the procedure, in contrast to the case of term delivery, in which levels decrease rapidly after birth.

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DO - 10.1016/j.fertnstert.2006.11.143

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JO - Fertility and Sterility

JF - Fertility and Sterility

IS - 3

ER -

Short-term clearance of cell-free nucleic acids after first-trimester termination of pregnancy

Abstract

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