Shifts in contractile regulatory protein subunits troponin T and troponin I in cardiac hypertrophy

Jagdish Gulati, Arvind Babu Akella, Srdjan D. Nikolic, Vito Starc, Francis Siri

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

To examine the molecular basis of hypertrophied heart failure, we investigated the changes in cardiac contractile regulatory proteins. The guinea pigs were subjected to chronic pressure overload with aortic banding to induce ventricular hypertrophy, and in-situ pressure-volume relations were recorded together with biochemical characterizations to ascertain the contractile modifications. Immunoblots of left and right ventricular samples revealed four distinct troponin T isoforms, which underwent alterations during hypertrophy. The higher molecular weight bands TnT1 and TnT2 shifted towards the lower molecular weight isoforms TnT3 and TnT4. For TnI, a single prominent band was detected, whose intensity also increased with pump failure. The findings provide the first direct evidence of TnT and TnI shifts in an experimentally induced hypertrophied heart failure and has novel mechanistic implications for the future studies.

Original languageEnglish
Pages (from-to)384-390
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume202
Issue number1
DOIs
StatePublished - 15 Jul 1994
Externally publishedYes

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