SH2D1A expression in Burkitt lymphoma cells is restricted to EBV positive group I lines and is downregulated in parallel with immunoblastic transformation

Noémi Nagy, Akihiko Maeda, Kentaro Bandobashi, Loránd L. Kis, Jun Nishikawa, Pankaj Trivedi, Alberto Faggioni, George Klein, Eva Klein

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

The SH2 domain containing SH2D1A protein has been characterized in relation to the X-linked lymphoproliferative disease (XLP), a primary immunodeficiency that leads to serious clinical conditions after Epstein-Barr virus (EBV) infection. The SH2D1A gene is mutated in the majority of XLP patients. We previously detected SH2D1A in activated T and NK cells, but not in B lymphocytes. We have found SH2D1A protein in Burkitt lymphoma (BL) lines, but only in those that carried EBV and had a Group I (germinal center) phenotype. All the EBV-carrying Group III (immunoblastic) and the EBV-negative BL lines tested were SH2D1A-negative. Motivated by these differences, we studied the impact of EBV and the cellular phenotype on SH2D1A expression. We approached the former question with BL sublines after both the loss of the virus and subsequent reinfection. We also tested original EBV-negative BL lines carrying transfected EBV genes, such as EBNA1, EBNA2, EBNA6, EBER1, 2 and LMP1, respectively. In our experiments, no direct relationship could be seen between EBV and SH2D1A expression. We modified the phenotype of the Group I BL cells by LMP1 transfection or CD40 ligation. The phenotypic changes, indicated by expression of immunoblastic markers, e.g., SLAM, were accompanied by downregulation of SH2D1A. It seems, therefore, that the presence of EBV and the phenotype of the cell together regulate SH2D1A expression in the BL cells. It is possible that SH2D1A is expressed in a narrow window of B cell development represented by germinal center cells.

Original languageEnglish
Pages (from-to)433-440
Number of pages8
JournalInternational Journal of Cancer
Volume100
Issue number4
DOIs
StatePublished - 1 Aug 2002
Externally publishedYes

Keywords

  • Activation
  • Burkitt lymphoma lines
  • Epstein-Barr virus
  • Phenotype
  • SH2D1A

Fingerprint

Dive into the research topics of 'SH2D1A expression in Burkitt lymphoma cells is restricted to EBV positive group I lines and is downregulated in parallel with immunoblastic transformation'. Together they form a unique fingerprint.

Cite this