Sexually dimorphic effects of hippocampal cholinergic deafferentation in rats

Zachariah Jonasson, Jonathan F.X. Cahill, Rachel E. Tobey, Mark G. Baxter

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

To determine whether the basal forebrain-hippocampal cholinergic system supports sexually dimorphic functionality, male and female Long-Evans rats were given either selective medial septum/vertical limb of the diagonal band (MS/VDB) cholinergic lesions using the neurotoxin 192 IgG-saporin or a control surgery and then postoperatively tested in a set of standard spatial learning tasks in the Morris water maze. Lesions were highly specific and effective as confirmed by both choline acetyltransferase/parvalbumin immunostaining and acetylcholinesterase histochemistry. Female controls performed worse than male controls in place learning and MS/VDB lesions failed to impair spatial learning in male rats, both consistent with previous findings. In female rats, MS/VDB cholinergic lesions facilitated spatial reference learning. A subsequent test of learning strategy in the water maze revealed a female bias for a response, relative to a spatial, strategy; MS/VDB cholinergic lesions enhanced the use of a spatial strategy in both sexes, but only significantly so in males. Together, these results indicate a sexually dimorphic function associated with MS/VDB-hippocampal cholinergic inputs. In female rats, these neurons appear to support sex-specific spatial learning processes.

Original languageEnglish
Pages (from-to)3041-3053
Number of pages13
JournalEuropean Journal of Neuroscience
Volume20
Issue number11
DOIs
StatePublished - Dec 2004
Externally publishedYes

Keywords

  • Acetylcholine
  • Basal forebrain
  • Reference memory
  • Saporin
  • Sex differences

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