Serotonin produces a reversible concentration dependent decrease of population spikes in rat hippocampal slices

Sheryl G. Beck; Joseph Goldfarb

doi:10.1016/0024-3205(85)90637-X

Serotonin produces a reversible concentration dependent decrease of population spikes in rat hippocampal slices

Sheryl G. Beck, Joseph Goldfarb

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Superfusion of slices of rat dorsal hippocampus in vitro with serotonin (5-HT) reversibly decreases the amplitude of the CAl population spike evoked by stimulation of stratum radiatum. The magnitude of the drug effect is concentration dependent and is greater on submaximal than on maximal population spikes. Concentration-response curves were generated using population spikes 30-60% of maximum by superfusion with increasing concentrations of 5-HT interspersed with perfusion of drug free medium. The EC₅₀ for 5-HT was 3.2uM. Repeated applications of a maximal dose of 5-HT did not produce techyphylaxis. The reversibility, reproducibility and concentration dependence of the 5-HT response in the rat hippocampal slice makes this preparation useful for rigorous pharmacological classification of the receptor(s) involved.

Original language	English
Pages (from-to)	557-563
Number of pages	7
Journal	Life Sciences
Volume	36
Issue number	6
DOIs	https://doi.org/10.1016/0024-3205(85)90637-X
State	Published - 11 Feb 1985
Externally published	Yes

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10.1016/0024-3205(85)90637-X

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title = "Serotonin produces a reversible concentration dependent decrease of population spikes in rat hippocampal slices",

abstract = "Superfusion of slices of rat dorsal hippocampus in vitro with serotonin (5-HT) reversibly decreases the amplitude of the CAl population spike evoked by stimulation of stratum radiatum. The magnitude of the drug effect is concentration dependent and is greater on submaximal than on maximal population spikes. Concentration-response curves were generated using population spikes 30-60% of maximum by superfusion with increasing concentrations of 5-HT interspersed with perfusion of drug free medium. The EC50 for 5-HT was 3.2uM. Repeated applications of a maximal dose of 5-HT did not produce techyphylaxis. The reversibility, reproducibility and concentration dependence of the 5-HT response in the rat hippocampal slice makes this preparation useful for rigorous pharmacological classification of the receptor(s) involved.",

author = "Beck, {Sheryl G.} and Joseph Goldfarb",

note = "Funding Information: The work was supported by USPHS grants RO3-MH39004 from the National Institute of Mental Health (to SGB) and DA01875 from the National Institute on Dru~ Abuse. SGB is a Revson Fellow supported by an award from the Revson Foundation. Curve fitting and analyses were done on the PROPHET Computer System, a national resource supported by the Chemical-Biological Information Handling Program, Division of Research Resources, National Institutes Health.",

year = "1985",

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doi = "10.1016/0024-3205(85)90637-X",

language = "English",

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TY - JOUR

T1 - Serotonin produces a reversible concentration dependent decrease of population spikes in rat hippocampal slices

AU - Beck, Sheryl G.

AU - Goldfarb, Joseph

N1 - Funding Information: The work was supported by USPHS grants RO3-MH39004 from the National Institute of Mental Health (to SGB) and DA01875 from the National Institute on Dru~ Abuse. SGB is a Revson Fellow supported by an award from the Revson Foundation. Curve fitting and analyses were done on the PROPHET Computer System, a national resource supported by the Chemical-Biological Information Handling Program, Division of Research Resources, National Institutes Health.

PY - 1985/2/11

Y1 - 1985/2/11

N2 - Superfusion of slices of rat dorsal hippocampus in vitro with serotonin (5-HT) reversibly decreases the amplitude of the CAl population spike evoked by stimulation of stratum radiatum. The magnitude of the drug effect is concentration dependent and is greater on submaximal than on maximal population spikes. Concentration-response curves were generated using population spikes 30-60% of maximum by superfusion with increasing concentrations of 5-HT interspersed with perfusion of drug free medium. The EC50 for 5-HT was 3.2uM. Repeated applications of a maximal dose of 5-HT did not produce techyphylaxis. The reversibility, reproducibility and concentration dependence of the 5-HT response in the rat hippocampal slice makes this preparation useful for rigorous pharmacological classification of the receptor(s) involved.

AB - Superfusion of slices of rat dorsal hippocampus in vitro with serotonin (5-HT) reversibly decreases the amplitude of the CAl population spike evoked by stimulation of stratum radiatum. The magnitude of the drug effect is concentration dependent and is greater on submaximal than on maximal population spikes. Concentration-response curves were generated using population spikes 30-60% of maximum by superfusion with increasing concentrations of 5-HT interspersed with perfusion of drug free medium. The EC50 for 5-HT was 3.2uM. Repeated applications of a maximal dose of 5-HT did not produce techyphylaxis. The reversibility, reproducibility and concentration dependence of the 5-HT response in the rat hippocampal slice makes this preparation useful for rigorous pharmacological classification of the receptor(s) involved.

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U2 - 10.1016/0024-3205(85)90637-X

DO - 10.1016/0024-3205(85)90637-X

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SN - 0024-3205

VL - 36

SP - 557

EP - 563

JO - Life Sciences

JF - Life Sciences

IS - 6

ER -

Serotonin produces a reversible concentration dependent decrease of population spikes in rat hippocampal slices

Abstract

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