Abstract
Serological and immunochemical studies showed that monoclonal antibody Q2/70 (MoAb Q2/70), produced by the hybridoma technique, is specific for human Ia-like antigens. This antibody recognizes an antigenic determinant which is different from those defining the serologic polymorphism of Ia-like antigens, and is expressed on subsets of human Ia-like molecules and on lymphoid cells from other species. MoAb Q2/70 inhibits unidirectional MLRs∗ between allogenic human lymphocytes, but not between murine and human lymphocytes. In ADCC∗ assays, MoAb Q2/70 mediates lysis of cultured human B lymphoid cells RPMI 4098, effected by murine splenocytes. The antibody is suitable to isolate immunologically functional B lymphocytes from human peripheral blood.
| Original language | English |
|---|---|
| Pages (from-to) | 211-223 |
| Number of pages | 13 |
| Journal | Human Immunology |
| Volume | 1 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1980 |
| Externally published | Yes |
Keywords
- ADCC
- AET-ShE
- Ab-E
- E
- Fab-E
- MLRs
- MoAb
- OxE
- PBL
- Protein A from Staphylococcus aureus
- SACI
- SDS-PAGE
- ShE
- SpA
- SpA-E
- Staphylococcus aureus Covan I strain
- antibody-dependent cellular cytotoxicity
- erythrocytes
- human peripheral blood lymphocytes
- mixed lymphocyze reactions
- monoclonal antibodies
- ox erythrocytes
- sheep erythrocutes chemically coated with Protein A from Staphylococcus aureus
- sheep erythrocytes
- sheep erythrocytes chemically coated with purified rabbit anti-mouse Ig antibodies
- sheep erythrocytes treated with 2-aminoethylisothiouronium bromide
- sheep erythrocytesm chemically coated with purified rabbit anti-mouse Fab antibodies
- sodium dodecyl sulphate-polyacrylamide gel electrophoresis
- β -microglubulin
- β -μ