TY - JOUR
T1 - Serological definition of individually distinct surface antigens in mouse mammary carcinomas
AU - Kuzumaki, Noboru
AU - Hilgers, Jo
AU - Klein, George
PY - 1980/12
Y1 - 1980/12
N2 - For serological analysis of surface antigens in mouse mammary carcinoma, 13 cultured lines were established from spontaneous and MTV-induced tumors of 6 different mouse strains. Six of the cultured lines, SHK of C3H, Ta3Ha and TA3St of A, S2Y of ABY, SBfnHB and SBfnHD of C3H foster-nursed CBA origin were highly sensitive to polyvalent anti-MTV and anti-MTV gp52 sera, and this was invariably correlated with the presence of MTVgp52 and p27 antigens in the cell extracts. All cultured lines were sensitive to anti-MuLVgp70 serum, and several lines also contained considerable amounts of MuLVp30 antigen in the cell extracts. By immunization with cultured or in vivo passaged tumor cells, 5 of 10 lines tested, SHF of C3H, S3W of ASW, S2Y of ABY; and SBfnHA and SBfnHB of C3H foster-nursed CBA origin could raise antisera containing complement-dependent cytotoxic antibodies to them in syngeneic or semisyngeneic mice. All the antisera contained antibodies against multiple specificities, although they were not cross-reactive to normal cells of C3H and BALB/c fetal origin, and normal spleen cells of C3H, DBA/2 and C57BL origin. One specificity of anti-S3W, anti-S2Y, anti-SBfnHA and anti-SBfnHB sera is cross-reactive for several leukemias and sarcomas, and the second specificity of anti-S3W, anti-SBfnHA and anti-SBfnHB sera is cross-reactive for polyoma virus-induced tumors. These antisera, after intensive absorption with leukemias and polyoma virus-induced tumor cells, and anti-SHF serum, demonstrated the third specificity which is cross-reactive for other mammary carcinomas and a C3H sarcoma. Further absorption with mammary carcinomas showed that anti-S3W and anti-SBfnHA sera have a fourth specificity, which had an absolute restriction to the immunizing tumor cells. These results suggest that individually distinct surface antigens were detectable in mouse mammary carcinomas by serological assays after absorption with cross-reactive cell lines.
AB - For serological analysis of surface antigens in mouse mammary carcinoma, 13 cultured lines were established from spontaneous and MTV-induced tumors of 6 different mouse strains. Six of the cultured lines, SHK of C3H, Ta3Ha and TA3St of A, S2Y of ABY, SBfnHB and SBfnHD of C3H foster-nursed CBA origin were highly sensitive to polyvalent anti-MTV and anti-MTV gp52 sera, and this was invariably correlated with the presence of MTVgp52 and p27 antigens in the cell extracts. All cultured lines were sensitive to anti-MuLVgp70 serum, and several lines also contained considerable amounts of MuLVp30 antigen in the cell extracts. By immunization with cultured or in vivo passaged tumor cells, 5 of 10 lines tested, SHF of C3H, S3W of ASW, S2Y of ABY; and SBfnHA and SBfnHB of C3H foster-nursed CBA origin could raise antisera containing complement-dependent cytotoxic antibodies to them in syngeneic or semisyngeneic mice. All the antisera contained antibodies against multiple specificities, although they were not cross-reactive to normal cells of C3H and BALB/c fetal origin, and normal spleen cells of C3H, DBA/2 and C57BL origin. One specificity of anti-S3W, anti-S2Y, anti-SBfnHA and anti-SBfnHB sera is cross-reactive for several leukemias and sarcomas, and the second specificity of anti-S3W, anti-SBfnHA and anti-SBfnHB sera is cross-reactive for polyoma virus-induced tumors. These antisera, after intensive absorption with leukemias and polyoma virus-induced tumor cells, and anti-SHF serum, demonstrated the third specificity which is cross-reactive for other mammary carcinomas and a C3H sarcoma. Further absorption with mammary carcinomas showed that anti-S3W and anti-SBfnHA sera have a fourth specificity, which had an absolute restriction to the immunizing tumor cells. These results suggest that individually distinct surface antigens were detectable in mouse mammary carcinomas by serological assays after absorption with cross-reactive cell lines.
UR - http://www.scopus.com/inward/record.url?scp=0019199865&partnerID=8YFLogxK
U2 - 10.1016/0014-2964(80)90030-4
DO - 10.1016/0014-2964(80)90030-4
M3 - Article
C2 - 6164557
AN - SCOPUS:0019199865
SN - 0014-2964
VL - 16
SP - 1569
EP - 1581
JO - European Journal of Cancer (1965)
JF - European Journal of Cancer (1965)
IS - 12
ER -