Sequential trafficking of Env and Gag to HIV-1 T cell virological synapses revealed by live imaging

Lili Wang, Sudeh Izadmehr, Edwin Kamau, Xiang Peng Kong, Benjamin K. Chen

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Background: HIV infection is enhanced by cell adhesions that form between infected and uninfected T cells called virological synapses (VS). VS are initiated by an interaction between Env and CD4 on cell surfaces and result in the recruitment of virus assembly to the site of cell-cell contact. However, the recruitment of Env to the VS and its relationship to Gag recruitment is not well defined. Results: To study the trafficking of HIV-1 Env through the VS, we constructed a molecular clone of HIV carrying a green fluorescent protein-Env fusion protein called, HIV Env-isfGFP-ΔV1V2. The Env-isfGFP-ΔV1V2 fusion protein does not produce virus particles on its own, but can be rescued by cotransfection with full-length HIV constructs and produce virus particles that package the fluorescent Env. These rescued fluorescent Env can participate in VS formation and can be used to directly image CD4-dependent Env transfer across VS from donor to target cells. The movements of fluorescently tagged Gag and Env to the VS and transfer into target cells can be also tracked through live imaging. Time lapse live imaging reveals evidence of limited Env accumulation at the site of cell-cell contact shortly after cell adhesion, followed by Gag re-distribution to contact area. Both Gag and Env can be recruited to form button-like spots characteristic of VS. Conclusions: Env and Gag are recruited to the VS in a coordinated temporal sequence and subsequently transfer together across the synapse into the target cell. Env accumulations, when observed, are earlier than Gag re-distribution to the contact area during formation of VS.

Original languageEnglish
Article number2
JournalRetrovirology
Volume16
Issue number1
DOIs
StatePublished - 15 Jan 2019

Keywords

  • Envelope protein
  • GFP
  • Gag
  • HIV-1
  • Virological synapse

Fingerprint

Dive into the research topics of 'Sequential trafficking of Env and Gag to HIV-1 T cell virological synapses revealed by live imaging'. Together they form a unique fingerprint.

Cite this